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Mutations of Phosphorylation Sites in MSL1 Protein Do Not Affect Dosage Compensation in Drosophila melanogaster

Abstract

Proteins MSL1 and MSL2 form the core of the Drosophila dosage compensation complex, which specifically binds to the X chromosome of males. Phosphorylation of certain amino acid residues was previously shown to regulate MSL1 activity. In the present work, transgenic lines of Drosophila expressing mutant variants of the MSL1 protein were obtained, in which amino acids undergoing phosphorylation were replaced. As a result, it was shown that inactivation of phosphorylation sites does not affect the efficiency of specific binding of the dosage compensation complex to the X chromosome of males and its functional activity.

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Funding

The study was supported by the Russian Science Foundation (project no. 17-74-20155).

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Correspondence to O. G. Maksimenko.

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Conflict of interest. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Translated by M. Batrukova

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Babosha, V.A., Georgiev, P.G. & Maksimenko, O.G. Mutations of Phosphorylation Sites in MSL1 Protein Do Not Affect Dosage Compensation in Drosophila melanogaster. Dokl Biochem Biophys 499, 225–227 (2021). https://doi.org/10.1134/S1607672921040025

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Keywords:

  • dosage compensation
  • Drosophila
  • MSL1
  • polytene chromosomes
  • transcription regulation
  • phosphorylation