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The Synthesis of Main Capsid Protein of Anogenital Type HPV6 L1 in Plant Expression System on the Basis of Tomato Fruits

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Abstract

The anogenital type HPV6 L1 major capsid protein was synthesized in a plant expression system on the basis of tomato fruits. The content of HPV6 L1 reached 380 μg per 1 mg of total soluble protein of raw fruit mass, which was represented as a single band with a molecular mass of 56 kDa on the SDS electrophoregram. When orally administrated to mice, the vaccine material from the tomato fruit transgenic for HPV6 L1 induced highly effective antibody immune response with a high titer. The cross-reactivity during the interaction of the antibody to the HPV6 L1 protein from peripheral blood serum of mice vaccinated with HPV6 L1 with the antigenic proteins HPV16 L1, HPV18 L1, HPV31 L1, and HPV45 L1 was found. This is promising for creating a vaccine with a broad reactivity against dangerous anogenital papillomatoses and cervical cancer.

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Authors and Affiliations

  1. Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences, Irkutsk, Russia

    N. I. Rekoslavskaya,  R. K. Salyaev & A. S. Stolbikov

  2. Irkutsk Scientific Centre, Irkutsk, Russia

    N. I. Rekoslavskaya

Authors
  1. N. I. Rekoslavskaya
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  2. R. K. Salyaev
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  3. A. S. Stolbikov
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Corresponding author

Correspondence to N. I. Rekoslavskaya.

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Conflict of interest. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. Work with animals was approved at a meeting of the Bioethical Council of the Siberian Institute of Plant Physiology and Biochemistry (Siberian Branch, Russian Academy of Sciences), Protocol no. 9 of October 30, 2019. Mice were housed in accordance with the standard conditions listed in GOST 33215-2014 and 33216-2014.

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Translated by M. Batrukova

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Rekoslavskaya, N.I., Salyaev, R.K. & Stolbikov, A.S. The Synthesis of Main Capsid Protein of Anogenital Type HPV6 L1 in Plant Expression System on the Basis of Tomato Fruits. Dokl Biochem Biophys 498, 193–198 (2021). https://doi.org/10.1134/S160767292103008X

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  • DOI: https://doi.org/10.1134/S160767292103008X

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