For in vitro fertilization technology, the quality of oocytes has a direct impact on the egg fertilization and developmental competence of early embryo. The morphological criteria are used for the estimation of oocyte quality before its fertilization in vitro. To date, only one method is known to determine the maturity of oocyte. This is the routine observation with a light microscope. The aim of this article was to adapt the noninvasive quantitative laser scanning microtomography (QLSM) for the investigation of morphological features of a human oocyte in vitro. This approach was used to accumulate the Z-stack gallery of optical sections of IVF oocyte. The layer-by-layer acquisition allows the fine cytoplasmic structure imaging. Applying the QLSM Z-stack of optical sections, the cellular volume was calculated with quantitative 3D reconstruction of a human oocyte. The volume value and intracellular structure were used as novel criteria to assess the oocyte state after the stress evoked by cryopreservation procedure.
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The part of the study devoted to the development of methods for studying the structure of micro-objects was supported by the Russian Science Foundation (project no. 20-16-00019).
Conflict of interest. The authors declare that they have no conflict of interest.
Statement of compliance with standards of research involving humans as subjects. The use of female germ cells for biomedical purposes is permitted by the ethics committee of the Kulakov Research Center for Obstetrics, Gynecology, and Perinatology of the Ministry of Health of the Russian Federation.
Translated by M. Batrukova
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Pogorelov, A.G., Makarova, N.P., Kalinina, E.A. et al. Laser Microtomography for IVF Oocyte of Human. Dokl Biochem Biophys 498, 190–192 (2021). https://doi.org/10.1134/S1607672921030078
- human mature oocyte
- quantitative laser microtomography (QLSM)
- in vitro fertilization technology (IVF)