Using the recombinant second fragment of the extracellular domain (EC2) of human desmoglein type 3 (Dsg3) as an affinity ligand, an immunosorbent was obtained that selectively binds autoreactive antibodies to this domain from the immune sera of patients with pemphigus. The EC2 protein was obtained in the form of a fusion protein with the Fc-fragment of human IgG1. The production was carried out in CHO cells using the method of transient expression.
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Koga, H., Tsuruta, D., Ohyama, B., Ishii, N., Hamada, T., Ohata, Ch., Furumura, M., and Hashimoto, T., Expert Opin. Ther. Targets, 2013, vol. 3, pp. 293–306.
Wan, H. and Choi, S., Encyclopedia of Signaling Molecules, Cham: Springer, 2016. https://doi.org/10.1007/978-1-4614-6438-9_101583-1
Futei, Y., Amagai, M., Sekiguchi, M., Nishifuji, K., Fujii, Y., and Nishikawa, T., J. Invest. Dermatol., 2000, vol. 115, pp. 829–834.
Sekiguchi, M., Futei, Y., Fujii, Y., Iwasaki, T., Nishikawa, T., and Amagai, M., J. Immunol., 2001, vol. 167, pp. 5439–5448.
Gosudarstvennaya Farmakopeya Rossiiskoi Federatsii, FS.3.3.2.0001.15 “Plazma cheloveka dlya fraktsionirovaniya”(Russian Pharmacopoeia. PM.3.3.2.0001.15 Human Plasma for Fractionation), 14th ed., Moscow, 2018, vol. 4.
This work was supported by the Ministry of Science and Higher Education of the Russian Federation (Agreement no. 075-15-2019-1942, unique project identifier RFMEFI60719X0325).
The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.
Translated by M. Batrukova
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Larina, E.N., Karasev, V.S., Shpilevaya, M.V. et al. Recombinant Fragment of the Extracellular Domain of Human Desmoglein 3 Fused with the Fc-Fragment of Human IgG1 Selectively Adsorbs Autoreactive Antibodies from the Sera of Pemphigus Patients. Dokl Biochem Biophys 498, 180–183 (2021). https://doi.org/10.1134/S1607672921030054
- human Dsg3
- CHO expression