Skip to main content

Recombinant Fragment of the Extracellular Domain of Human Desmoglein 3 Fused with the Fc-Fragment of Human IgG1 Selectively Adsorbs Autoreactive Antibodies from the Sera of Pemphigus Patients

Abstract

Using the recombinant second fragment of the extracellular domain (EC2) of human desmoglein type 3 (Dsg3) as an affinity ligand, an immunosorbent was obtained that selectively binds autoreactive antibodies to this domain from the immune sera of patients with pemphigus. The EC2 protein was obtained in the form of a fusion protein with the Fc-fragment of human IgG1. The production was carried out in CHO cells using the method of transient expression.

This is a preview of subscription content, access via your institution.

Fig. 1.

REFERENCES

  1. 1

    Koga, H., Tsuruta, D., Ohyama, B., Ishii, N., Hamada, T., Ohata, Ch., Furumura, M., and Hashimoto, T., Expert Opin. Ther. Targets, 2013, vol. 3, pp. 293–306.

    Article  Google Scholar 

  2. 2

    Wan, H. and Choi, S., Encyclopedia of Signaling Molecules, Cham: Springer, 2016. https://doi.org/10.1007/978-1-4614-6438-9_101583-1

    Book  Google Scholar 

  3. 3

    Futei, Y., Amagai, M., Sekiguchi, M., Nishifuji, K., Fujii, Y., and Nishikawa, T., J. Invest. Dermatol., 2000, vol. 115, pp. 829–834.

    CAS  Article  Google Scholar 

  4. 4

    Sekiguchi, M., Futei, Y., Fujii, Y., Iwasaki, T., Nishikawa, T., and Amagai, M., J. Immunol., 2001, vol. 167, pp. 5439–5448.

    CAS  Article  Google Scholar 

  5. 5

    Gosudarstvennaya Farmakopeya Rossiiskoi Federatsii, FS.3.3.2.0001.15 “Plazma cheloveka dlya fraktsionirovaniya”(Russian Pharmacopoeia. PM.3.3.2.0001.15 Human Plasma for Fractionation), 14th ed., Moscow, 2018, vol. 4.

Download references

Funding

This work was supported by the Ministry of Science and Higher Education of the Russian Federation (Agreement no. 075-15-2019-1942, unique project identifier RFMEFI60719X0325).

Author information

Affiliations

Authors

Corresponding author

Correspondence to E. N. Larina.

Ethics declarations

The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

Additional information

Translated by M. Batrukova

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Larina, E.N., Karasev, V.S., Shpilevaya, M.V. et al. Recombinant Fragment of the Extracellular Domain of Human Desmoglein 3 Fused with the Fc-Fragment of Human IgG1 Selectively Adsorbs Autoreactive Antibodies from the Sera of Pemphigus Patients. Dokl Biochem Biophys 498, 180–183 (2021). https://doi.org/10.1134/S1607672921030054

Download citation

Keywords:

  • pemphigus
  • human Dsg3
  • autoantibody
  • CHO expression
  • immunoadsorption