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TTK Isoforms Interact with Two Regions of the Mep-1 Protein of Drosophila melanogaster

Abstract

The Drosophila TTK protein is involved in the processes of cell differentiation and is represented by two isoforms, TTK69 and TTK88, which have a common N-terminal BTB domain and different C-terminal sequences. Earlier, it was shown that TTK69 represses the activity of enhancers and promoters by recruiting a conserved among higher eukaryotes NURD complex to chromatin. The Mep-1 protein was found in the NURD-complex of Drosophila, and this protein can interact with the C-terminal region of TTK69. In the present study, using the yeast two-hybrid system, we mapped the interacting regions of the TTK and Mep-1 proteins. We identified regions in the unique C-terminal regions of TTK isoforms that can interact simultaneously with two regions of the Mep-1 protein. The results show that, despite the low homology of the C-terminal regions, the TTK isoform retains the ability to interact with two conserved regions of the Mep-1 protein, which suggests the functional significance of this interaction.

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Funding

The study was supported by the Russian Foundation for Basic Research (project no. 18-04-01272).

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Correspondence to O. G. Maksimenko.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by M. Batrukova

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Khalisova, K.Y., Osadchiy, I.S., Georgiev, P.G. et al. TTK Isoforms Interact with Two Regions of the Mep-1 Protein of Drosophila melanogaster . Dokl Biochem Biophys 498, 177–179 (2021). https://doi.org/10.1134/S1607672921030042

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Keywords:

  • NURD complex
  • transcriptional repression
  • protein–protein interactions