Skip to main content

TTK Isoforms Interact with Two Regions of the Mep-1 Protein of Drosophila melanogaster


The Drosophila TTK protein is involved in the processes of cell differentiation and is represented by two isoforms, TTK69 and TTK88, which have a common N-terminal BTB domain and different C-terminal sequences. Earlier, it was shown that TTK69 represses the activity of enhancers and promoters by recruiting a conserved among higher eukaryotes NURD complex to chromatin. The Mep-1 protein was found in the NURD-complex of Drosophila, and this protein can interact with the C-terminal region of TTK69. In the present study, using the yeast two-hybrid system, we mapped the interacting regions of the TTK and Mep-1 proteins. We identified regions in the unique C-terminal regions of TTK isoforms that can interact simultaneously with two regions of the Mep-1 protein. The results show that, despite the low homology of the C-terminal regions, the TTK isoform retains the ability to interact with two conserved regions of the Mep-1 protein, which suggests the functional significance of this interaction.

This is a preview of subscription content, access via your institution.

Fig. 1.
Fig. 2.


  1. 1

    Harrison, S.D. and Travers, A.A., EMBO J., 1990, vol. 9, pp. 207–216.

    CAS  Article  Google Scholar 

  2. 2

    Brown, J.L. and Wu, C., Development, 1993, vol. 117, pp. 45–58.

    CAS  Article  Google Scholar 

  3. 3

    Read, D. and Manley, J.L., EMBO J., 1992, vol. 11, pp. 1035–1044.

    CAS  Article  Google Scholar 

  4. 4

    Ciglar, L., Girardot, C., Wilczynski, B., et al., Development, 2014, vol. 141, pp. 2633–2643.

    CAS  Article  Google Scholar 

  5. 5

    Badenhorst, P., Finch, J.T., and Travers, A.A., Mech. Dev., 2002, vol. 117, pp. 87–101.

    CAS  Article  Google Scholar 

  6. 6

    Lai, Z.C., Harrison, S.D., Karim, F., et al., Proc. Natl. Acad. Sci. U. S. A., 1996, vol. 93, pp. 5025–5030.

    CAS  Article  Google Scholar 

  7. 7

    Bonchuk, A., Denisov, S., Georgiev, P., et al., J. Mol. Biol., 2011, vol. 412, pp. 423–436.

    CAS  Article  Google Scholar 

  8. 8

    Kunert, N., Wagner, E., Murawska, M., et al., EMBO J., 2009, vol. 28, pp. 533–544.

    CAS  Article  Google Scholar 

  9. 9

    Bracken, A.P., Brien, G.L., and Verrijzer, C.P., Genes Dev., 2019, vol. 33, pp. 936–959.

    CAS  Article  Google Scholar 

  10. 10

    Reddy, B.A., Bajpe, P.K., Bassett, A., et al., Mol. Cell Biol., 2010, vol. 30, pp. 5234–5244.

    CAS  Article  Google Scholar 

Download references


The study was supported by the Russian Foundation for Basic Research (project no. 18-04-01272).

Author information



Corresponding author

Correspondence to O. G. Maksimenko.

Ethics declarations

The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

Additional information

Translated by M. Batrukova

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Khalisova, K.Y., Osadchiy, I.S., Georgiev, P.G. et al. TTK Isoforms Interact with Two Regions of the Mep-1 Protein of Drosophila melanogaster . Dokl Biochem Biophys 498, 177–179 (2021).

Download citation


  • NURD complex
  • transcriptional repression
  • protein–protein interactions