Abstract
The current study evaluated Saussurea lappa extract (SLE) as possible therapeutic agent for osteoarthritis treatment in mice model. Male BALB/c nude mice were separated into sham, model and SLE treatment (at 1.0, 1.5, 2.0, 2.5, and 3.0 mg/kg) groups. Osteoarthritis mice model was prepared by injecting 5 mg/kg doses of monosodium iodoacetate (MIA) to mice via intra-articular route. The SLE was injected to mice for 20 days from day 2 of MIA injection through intraperitoneal route. The SLE treatment alleviated OA-induced higher secretion of interleukin-6, TNF-α, and IL-1β in mice serum. Moreover, elevated levels of P2X7R and MMP-13 in OA mice were also significantly down-regulated on treatment with SLE. In OA mice SLE treatment suppressed expression of SP and PGE2 in cartilage tissues. The expression of activated IκBα and NF-κB p65 was also inhibited markedly by SLE treatment in OA mice. In summary, SLE has protective effect on osteoarthritis in mice by targeting interleukin overproduction and P2X7R expression. Besides, it suppressed MMP expression and deactivated NF-κB signaling pathway. Therefore, SLE can be developed for the treatment of osteoarthritis.
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We declare that this work was done by the authors named in this article and all liabilities pertaining to claims relating to the content of this article will be borne by the authors. Xiaohu Zhu designed the study and wrote the paper. Dong Feng and Xiaohu Zhu performed the experimental work, carried out the literature study, analyzed and compiled the data. The research article was thoroughly read by all the authors before communication for the consideration of publication.
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Approval to conduct present study was given by the Ethics Committee of Xi’an Hospital (Shaanxi, China). All experimental protocols were performed as per the guidelines issued by the National Institute of Health, China.
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Feng, D., Zhu, X. Preventive Effect of Saussurea lappa Extract on Osteoarthritis in Mice Model through Inhibition of NF-κB Pathway. Dokl Biochem Biophys 496, 56–61 (2021). https://doi.org/10.1134/S1607672921010142
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DOI: https://doi.org/10.1134/S1607672921010142