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Novel pH-Sensitive Microbial Rhodopsin from Sphingomonas paucimobilis

  • BIOCHEMISTRY, BIOPHYSICS, AND MOLECULAR BIOLOGY
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An Erratum to this article was published on 01 March 2021

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Abstract

This work provides the first characteristics of the rhodopsin SpaR from Sphingomonas paucimobilis, aerobic bacteria associated with opportunistic infections. The sequence analysis of SpaR has shown that this protein has unusual DTS motif which has never reported in rhodopsins from Proteobacteria. We report that SpaR operates as an outward proton pump at low pH; however, proton pumping is almost absent at neutral and alkaline pH. The photocycle of this rhodopsin in detergent micelles slows down with an increase in pH because of longer Schiff base reprotonation. Our results show that the novel microbial ion transporter SpaR of interest both as an object for basic research of membrane proteins and as a promising optogenetic tool.

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Funding

This work was partially supported by the Russian Foundation for Basic Research, project no. 17-00-00167K (KOMFI 17-00-00164, 17-00-00165, 17-00-00166).

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Authors and Affiliations

  1. Moscow Institute of Physics and Technology, Dolgoprudnyi, Moscow oblast, Russia

    N. Maliar, I. S. Okhrimenko, A. A. Alekseev, K. V. Kovalev, D. V. Soloviov, P. A. Popov, D. V. Zabelskii & V. I. Gordeliy

  2. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia

    L. E. Petrovskaya, D. A. Dolgikh &  M. P. Kirpichnikov

  3. Institut de Biologie Structurale (IBS), Université Grenoble Alpes, CEA, CNRS, Grenoble, France

    K. V. Kovalev & V. I. Gordeliy

  4. Institute of Biological Information Processing (IBI-7: Structural Biochemistry), Forschungszentrum Jülich GmbH, Jülich, Germany

    A. A. Alekseev, K. V. Kovalev, D. V. Zabelskii & V. I. Gordeliy

  5. JuStruct: Jülich Center for Structural Biology, Forschungszentrum Jülich GmbH, Jülich, Germany

    A. A. Alekseev, K. V. Kovalev, D. V. Zabelskii & V. I. Gordeliy

  6. Institute of Crystallography, University of Aachen (RWTH), Aachen, Germany

    A. A. Alekseev & K. V. Kovalev

  7. Institute for Safety Problems of Nuclear Power Plants, National Academy of Sciences of Ukraine, Kyiv, Ukraine

    D. V. Soloviov

  8. Joint Institute for Nuclear Research, Dubna, Russia

    D. V. Soloviov

  9. Skolkovo Institute of Science and Technology, Moscow, Russia

    P. A. Popov

  10. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russia

    T. I. Rokitskaya & Y. N. Antonenko

  11. Biological Faculty, Moscow State University, Moscow, Russia

    D. A. Dolgikh &  M. P. Kirpichnikov

Authors
  1. N. Maliar
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  2. I. S. Okhrimenko
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  3. L. E. Petrovskaya
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  4. A. A. Alekseev
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  5. K. V. Kovalev
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  6. D. V. Soloviov
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  7. P. A. Popov
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  8. T. I. Rokitskaya
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  9. Y. N. Antonenko
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  10. D. V. Zabelskii
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  11. D. A. Dolgikh
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  12. M. P. Kirpichnikov
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  13. V. I. Gordeliy
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Corresponding author

Correspondence to V. I. Gordeliy.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

AUTHOR CONTRIBUTIONS

N. Maliar, I.S. Okhrimenko, and L.E. Petrovskaya contributed equally to the work.

Additional information

Translated by M. Batrukova

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Maliar, N., Okhrimenko, I.S., Petrovskaya, L.E. et al. Novel pH-Sensitive Microbial Rhodopsin from Sphingomonas paucimobilis . Dokl Biochem Biophys 495, 342–346 (2020). https://doi.org/10.1134/S1607672920060162

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  • DOI: https://doi.org/10.1134/S1607672920060162

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