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New Sea Anemone Toxin RTX-VI Selectively Modulates Voltage-Gated Sodium Channels

  • BIOCHEMISTRY, BIOPHYSICS, AND MOLECULAR BIOLOGY
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Abstract

A new neurotoxin RTX-VI that modulates the voltage-gated sodium channels (NaV) was isolated from the ethanolic extract of the sea anemone Heteractis crispa. Its amino acid sequence was determined using the combination of Edman degradation and tandem mass spectrometry. RTX-VI turned out to be an unusual natural analogue of the previously described sea anemone toxin RTX-III. The RTX-VI molecule consists of two disulfide-linked peptide chains and is devoid of Arg13, which is important for the selectivity and affinity of such peptides for the NaV channels. Electrophysiological screening of RTV-VI on NaV channel subtypes showed its selective interaction with the central nervous system (NaV1.2, NaV1.6) and insect (BgNaV1, VdNaV1) sodium channels.

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ACKNOWLEDGMENTS

The study was carried out on the equipment of the Collective Facilities Center “The Far Eastern Center for Structural Molecular Research (NMR/MS) PIBOC FEB RAS.”

Funding

This work was supported by the Russian Foundation for Basic Research (grant no. 18-04-00631).

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Authors and Affiliations

  1. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia

    R. S. Kalina, I. N. Gladkikh, P. S. Dmitrenok, N. Y. Kim, E. V. Leychenko, M. M. Monastyrnaya & E. P. Kozlovskaya

  2. Department of Toxicology and Pharmacology, University of Leuven, Leuven, Belgium

    S. Peigneur & J. Tytgat

Authors
  1. R. S. Kalina
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  2. S. Peigneur
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  3. I. N. Gladkikh
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  4. P. S. Dmitrenok
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  5. N. Y. Kim
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  6. E. V. Leychenko
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  7. M. M. Monastyrnaya
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  8. J. Tytgat
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  9. E. P. Kozlovskaya
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Corresponding author

Correspondence to R. S. Kalina.

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Conflict of interest. The authors declare that the studies were conducted in the absence of any commercial or financial relationship that could be construed as a potential conflict of interest.

Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Translated by M. Batrukova

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Kalina, R.S., Peigneur, S., Gladkikh, I.N. et al. New Sea Anemone Toxin RTX-VI Selectively Modulates Voltage-Gated Sodium Channels. Dokl Biochem Biophys 495, 292–295 (2020). https://doi.org/10.1134/S1607672920060071

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  • DOI: https://doi.org/10.1134/S1607672920060071

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