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Inhibition of Histone Deacetylases 4 and 5 Reduces Titin Proteolysis and Prevents Reduction of TTN Gene Expression in Atrophied Rat Soleus Muscle after Seven-Day Hindlimb Unloading

  • BIOCHEMISTRY, BIOPHYSICS, AND MOLECULAR BIOLOGY
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Abstract

The effect of HDACs 4 and 5 on the level of atrophy, calpain-1 and titin content, and TTN gene expression in rat soleus after 7-day gravitational unloading (hindlimb suspension model) was studied. The development of atrophic changes induced by gravitational unloading in rat soleus was accompanied by an increase in the calpain-1 content, an increase in titin proteolysis, and a decrease in the mRNA content of the protein. Inhibition of HDACs 4 and 5 did not eliminate the development of unloading-induced atrophy but significantly prevented proteolysis of titin and the decrease in the TTN gene expression.

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ACKNOWLEDGMENTS

This work was performed using the equipment of the regional Pushchino Core Facility “Structural and Functional Studies of Biosystems” of the Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences.

Funding

This work was supported by the Russian Science Foundation (project no. 18-15-00062).

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Authors and Affiliations

  1. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia

    Yu. V. Gritsyna, A. D. Ulanova, S. S. Popova, A. G. Bobylev & I. M. Vikhlyantsev

  2. Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Russia

    V. K. Zhalimov

  3. Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia

    T. L. Nemirovskaya & B. S. Shenkman

Authors
  1. Yu. V. Gritsyna
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  2. A. D. Ulanova
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  3. S. S. Popova
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  4. A. G. Bobylev
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  5. V. K. Zhalimov
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  6. T. L. Nemirovskaya
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  7. B. S. Shenkman
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  8. I. M. Vikhlyantsev
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Corresponding author

Correspondence to I. M. Vikhlyantsev.

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Conflict of interest. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. The study was performed in accordance with the protocol no. 24/2019 of April 5, 2019, of the Commission on Biological Safety and Bioethics of the Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences.

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Translated by M. Batrukova

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Gritsyna, Y.V., Ulanova, A.D., Popova, S.S. et al. Inhibition of Histone Deacetylases 4 and 5 Reduces Titin Proteolysis and Prevents Reduction of TTN Gene Expression in Atrophied Rat Soleus Muscle after Seven-Day Hindlimb Unloading. Dokl Biochem Biophys 495, 338–341 (2020). https://doi.org/10.1134/S1607672920060058

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  • DOI: https://doi.org/10.1134/S1607672920060058

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