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Differences in the Drosha and Dicer Cleavage Profiles in Colorectal Cancer and Normal Colon Tissue Samples

Doklady Biochemistry and Biophysics volume 493, pages 208–210 (2020)Cite this article

Abstract

Human colorectal adenocarcinoma cell line Caco-2 is often used as a model of healthy intestinal epithelium, in particular, in miRNA studies. The work of the enzymes Drosha and Dicer is an integral part of the process of miRNA formation. Inaccuracies in the work of these enzymes lead to a change in the nucleotide sequences of miRNAs with the formation of new isoforms, which, in turn, can change intracellular regulatory mechanisms. In the framework of this study, it was shown that the quantitative estimates of inaccuracies in Drosha and Dicer activity significantly differ between the specimens of normal colon tissue and malignant colorectal tumors.

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REFERENCES

  1. Nikulin, S.V., Knyazev, E.N., Gerasimenko, T.N., et al., Bull. Exp. Biol. Med., 2018, vol. 166, no. 1, pp. 35–38.

    CAS  Article  Google Scholar 

  2. Nikulin, S.V., Knyazev, E.N., Gerasimenko, T.N., et al., Mol. Biol., 2019, vol. 53, no. 3, pp. 411–418.

    CAS  Article  Google Scholar 

  3. Bein, A., Shin, W., Jalili-Firoozinezhad, S., et al., Cell. Mol. Gastroenterol. Hepatol., 2018, vol. 5, no. 4, pp. 659–668.

    Article  Google Scholar 

  4. Knyazev, E.N., Samatov, T.R., Fomicheva, K.A., et al., Bull. Exp. Biol. Med., 2016, vol. 161, no. 1, pp. 112–115.

    CAS  Article  Google Scholar 

  5. Skurnikov, M.Y., Makarova, Y.A., Knyazev, E.N., et al., Bull. Exp. Biol. Med., 2016, vol. 160, no. 5, pp. 632–634.

    CAS  Article  Google Scholar 

  6. Lee, Y., Ahn, C., Han, J., et al., Nature, 2003, vol. 425, no. 6956, pp. 415–419.

    CAS  Article  Google Scholar 

  7. Hutvágner, G., McLachlan, J., Pasquinelli, A.E., et al., Science, 2001, vol. 293, no. 5531, pp. 834–838.

    Article  Google Scholar 

  8. Morin, R.D., O’Connor, M.D., Griffith, M., et al., Genome Res., 2008, vol. 18, no. 4, pp. 610–621.

    CAS  Article  Google Scholar 

  9. Loher, P., Londin, E.R., and Rigoutsos, I., Oncotarget, 2014, vol. 5, no. 18, pp. 8790–8802.

    Article  Google Scholar 

  10. Telonis, A.G., Loher, P., Jing, Y., et al., Nucleic Acids Res., 2015, vol. 43, no. 19, pp. 9158–9175.

    CAS  Article  Google Scholar 

  11. Mercey, O., Popa, A., Cavard, A., et al., FEBS Lett., 2017, vol. 591, no. 5, pp. 693–705.

    CAS  Article  Google Scholar 

  12. Cancer Genome Atlas Network, Nature, 2012, vol. 487, no. 7407, pp. 330–337.

    Article  Google Scholar 

  13. Murtagh, F. and Legendre, P., J. Classif., 2014, vol. 31, no. 3, pp. 274–295.

    Article  Google Scholar 

  14. Moisenovich, M., Tonevitsky, A., Maljuchenko, N., et al., Histochem. Cell Biol., 2004, vol. 121, no. 6, pp. 429–439.

    CAS  Article  Google Scholar 

  15. Samatov, T.R., Shkurnikov, M.U., Tonevitskaya, S.A., et al., Prog. Histochem. Cytochem., 2015, vol. 49, no. 4, pp. 21–29.

    Article  Google Scholar 

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Funding

The study was supported by the Russian Foundation for Basic Research (project no. 20-34-70092).

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Affiliations

  1. Hertsen Moscow Oncology Research Center, Branch of the National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, Russia

    M. Yu. Shkurnikov

  2. Faculty of Biology and Biotechnology, National Research University Higher School of Economics, Moscow, Russia

    S. A. Nersisyan & D. V. Maltseva

  3. Faculty of Mechanics and Mathematics, Moscow State University, Moscow, Russia

    S. A. Nersisyan & A. Sh. Osepyan

  4. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia

    D. V. Maltseva & E. N. Knyazev

Authors
  1. M. Yu. Shkurnikov
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  2. S. A. Nersisyan
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  3. A. Sh. Osepyan
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  4. D. V. Maltseva
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  5. E. N. Knyazev
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Corresponding authors

Correspondence to M. Yu. Shkurnikov or E. N. Knyazev.

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Conflict of interest. The authors declare that they have no conflict of interest.

Statement of compliance with standards of research involving humans as subjects. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants involved in the study.

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Translated by M. Batrukova

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Shkurnikov, M.Y., Nersisyan, S.A., Osepyan, A.S. et al. Differences in the Drosha and Dicer Cleavage Profiles in Colorectal Cancer and Normal Colon Tissue Samples. Dokl Biochem Biophys 493, 208–210 (2020). https://doi.org/10.1134/S1607672920040122

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  • DOI: https://doi.org/10.1134/S1607672920040122

Keywords:

  • Drosha
  • Dicer
  • colorectal cancer
  • miRNA
  • microRNA
  • microRNA isoform
  • isomiR