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Differential Expression of Bipotent Commitment-Related Genes in Multipotent Mesenchymal Stromal Cells at Different O2 Levels

  • BIOCHEMISTRY, BIOPHYSICS, AND MOLECULAR BIOLOGY
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Abstract

The transcriptomic profile associated with osteo- and adipogenic differentiation in growth-arrested multipotent mesenchymal stromal cells (MSCs) from human adipose tissue was analyzed in vitro at 20% (standard laboratory) and 5% (tissue-related) O2 levels. Compared with day 7, at 5% O2 on day 14 spontaneous upregulation of osteo- (RUNX2, SP7, BGLAP, and SPP1) and adipogenic differentiation (CEBPA, PPARG, and ADIPOQ) genes in MSCs was observed (p < 0.05). Thus, upon expansion under tissue-related O2, MSCs demonstrated a bipotent transcriptomic profile, which may contribute to the improvement of their hematopoiesis-supportive function.

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Funding

The study was supported by the Russian Foundation for Basic Research (project no. 19-29-04026) and the Program of the Presidium of the Russian Academy of Sciences no. 18 “Basic Research for Biomedical Technologies.”

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Authors and Affiliations

  1. Institute of Biomedical Problems, Russian Academy of Sciences, 123007, Moscow, Russia

    L. B. Buravkova, M. I. Ezdakova, I. V. Andrianova, E. A. Golikova & E. R. Andreeva

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  1. L. B. Buravkova
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  2. M. I. Ezdakova
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  3. I. V. Andrianova
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  4. E. A. Golikova
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  5. E. R. Andreeva
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Correspondence to L. B. Buravkova.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by M. Batrukova

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Buravkova, L.B., Ezdakova, M.I., Andrianova, I.V. et al. Differential Expression of Bipotent Commitment-Related Genes in Multipotent Mesenchymal Stromal Cells at Different O2 Levels. Dokl Biochem Biophys 491, 67–69 (2020). https://doi.org/10.1134/S1607672920020052

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  • DOI: https://doi.org/10.1134/S1607672920020052

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