Abstract
The material of patients with invasive carcinoma of no special type (ICNT) and nonmalignant diseases (ND) of the mammary gland was studied. When comparing the concentrations of histidine-rich glycoprotein (HRG) and E-cadherin (CDH1), statistically significant differences between ICNT and ND by HRG in the supernatant of blood cells and its spontaneous production by biopsies and by CDH1 at its induced production, as well as by influence indices of polyclonal activators on the production of CDH1 were found. When comparing the expression of immunohistochemical markers, no statistically significant differences between ICNT and ND were obtained.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Autenshlyus, A.I., Golovanova, A.V., Studenikina, A.A., Brusentsov, I.I., Proskura, A.V., Zhurakovskiy, I.P., Arkhipov, S.A., Sidorov, S.V., Vavilin, V.A., and Lyakhovich, V.V., Dokl. Biochem. Biophys., 2019, vol. 484, pp. 59–62.
Shukla, H.D., Mahmood, J., and Vujaskovic, Z., Integrated proteo-genomic approach for early diagnosis and prognosis of cancer, Cancer Lett, 2015, vol. 369, no. 1, pp. 28–36.
Zeidan, B.A., Townsend, P.A., Garbis, S.D., et al., Clinical proteomics and breast cancer, Surgeon, 2015, vol. 13, no. 5, pp. 271–278.
Ankney, J.A., Xie, L., Wrobel, J.A., et al., Novel secretome-to-transcriptome integrated or secreto-transcriptomic approach to reveal liquid biopsy biomarkers for predicting individualized prognosis of breast cancer patients, BMC Med. Genomics, 2019, vol. 12, no. 78, pp. 1–20.
Kumar, S., Das, A., and Sen, S., Extracellular matrix density promotes EMT by weakening cell-cell adhesions, Mol. Biosyst., 2014, vol. 10, no. 4, pp. 838–850.
Lin, J.H., Lee, W.J., Wu, H.C., et al., Small g protein signalling modulator 2 (SGSM2) is involved in oestrogen receptor-positive breast cancer metastasis through enhancement of migratory cell adhesion via interaction with E-cadherin, Cell Adh. Migr., 2019, vol. 13, no. 1, pp. 120–137.
Tang, X.B., Dong, P.L., Wang, J., et al., Effect of autologous platelet-rich plasma on the chondrogenic differentiation of rabbit adipose-derived stem cells in vitro, Exp. Ther. Med., 2015, vol. 10, no. 2, pp. 477–483.
Savci-Heijink, C.D., Halfwerk, H., Hooijer, G.K.J., et al., Epithelial-to-mesenchymal transition status of primary breast carcinomas and its correlation with metastatic behavior, Breast Cancer Res. Treat., 2019, vol. 174, no. 3, pp. 649–659.
Funding
This study was supported by the state assignment of the Ministry of Health of the Russian Federation.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest. The authors declare no explicit and potential conflicts of interest associated with the publication of this work.
Statement of compliance with standards of research involving humans as subjects. All studies were performed in accordance with the Helsinki Declaration, World Medical Association. The study was approved by the Local Ethics Committee of Novosibirsk State Medical University. Informed consent was obtained from all individual participants involved in the study.
Additional information
Translated by M. Batrukova
Rights and permissions
About this article
Cite this article
Autenshlyus, A.I., Bernado, A.V., Studenikina, A.A. et al. Personalized Approach to Determination of Histidine-Rich Glycoprotein and E-Cadherin in Supernatants of Immunocompetent Blood Cells and Breast Biopsy Specimens in Breast Malignant and Non-Malignant Disease. Dokl Biochem Biophys 490, 1–4 (2020). https://doi.org/10.1134/S1607672920010019
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1607672920010019