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Recombinant Analogue of the Human Protein SLURP-1 Inhibits the Growth of Multicellular Spheroids Reconstructed from Carcinoma Cells

  • BIOCHEMISTRY, BIOPHYSICS, AND MOLECULAR BIOLOGY
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Abstract

In the present study we showed that the recombinant analogue of the SLURP-1 protein effectively inhibits the growth of a 3D model of tumors—multicellular spheroids reconstructed from human epidermoid carcinoma A431 cells and human lung adenocarcinoma A549 cells. The combined application of rSLURP-1 with gefitinib (inhibitor of epidermal growth factor receptor (EGFR)) leads to the synergistic antiproliferative effect on spheroids from A431 cells. The results obtained suggest the possibility for design of first-in-class anticancer drugs based on recombinant SLURP-1.

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Funding

This work was supported by the Russian Science Foundation (project no. 17-74-20161).

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Authors and Affiliations

  1. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997, Moscow, Russia

    M. L. Bychkov, M. A. Shulepko, O. V. Shlepova, E. N. Lyukmanova &  M. P. Kirpichnikov

  2. Moscow Institute of Physics and Technology (National Research University), 141701, Dolgoprudnyi, Moscow oblast, Russia

    O. V. Shlepova & E. N. Lyukmanova

  3. Faculty of Biology, Moscow State University, 119234, Moscow, Russia

    M. P. Kirpichnikov

Authors
  1. M. L. Bychkov
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  2. M. A. Shulepko
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  3. O. V. Shlepova
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  4. E. N. Lyukmanova
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  5. M. P. Kirpichnikov
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Corresponding authors

Correspondence to M. A. Shulepko or E. N. Lyukmanova.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by M. Batrukova

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Bychkov, M.L., Shulepko, M.A., Shlepova, O.V. et al. Recombinant Analogue of the Human Protein SLURP-1 Inhibits the Growth of Multicellular Spheroids Reconstructed from Carcinoma Cells. Dokl Biochem Biophys 489, 392–395 (2019). https://doi.org/10.1134/S1607672919060103

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  • DOI: https://doi.org/10.1134/S1607672919060103

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