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NSP Protein Encoded in Negative NS RNA Strand of Influenza A Virus Induces Cellular Immune Response in Infected Animals

Doklady Biochemistry and Biophysics volume 486, pages 201–205 (2019)Cite this article

Abstract

Infection of mice with influenza A viruses led to the formation of clones of lymphocytes that specifically recognizes viral domains in the central zone of the NSP protein (amino acid positions 83–119). Computer analysis of the primary structure of the NSP protein showed the presence of T-cell epitopes in the central part of the NSP molecule. The findings indicate that the viral NSP gene is expressed in the infected animals and verify the concept of the bipolar strategy (ambisense strategy) of the influenza A virus genome.

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Funding

This work was partially supported by the Russian Foundation for Basic Research (project nos. 16-04-01271, 13-04-001824) and the Scientific Program SFB 293 of the German Research Society (DFG).

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Affiliations

  1. Ivanovsky Institute of Virology, Gamaleya Research Center of Epidemiology and Microbiology, Ministry of Healthcare of the Russian Federation, 123098, Moscow, Russia

    O. P. Zhirnov & E. I. Isaeva

  2. Russian–German Academy of Medical and Biotechnological Sciences, 142782, Moscow, Russia

    O. P. Zhirnov

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  1. O. P. Zhirnov
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  2. E. I. Isaeva
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Correspondence to O. P. Zhirnov.

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Conflict of interests. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Translated by M. Batrukova

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Zhirnov, O.P., Isaeva, E.I. NSP Protein Encoded in Negative NS RNA Strand of Influenza A Virus Induces Cellular Immune Response in Infected Animals. Dokl Biochem Biophys 486, 201–205 (2019). https://doi.org/10.1134/S1607672919030128

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  • DOI: https://doi.org/10.1134/S1607672919030128