Doklady Biochemistry and Biophysics

, Volume 485, Issue 1, pp 157–161 | Cite as

Comparison of Earthworm Bioluminescent Systems

  • N. S. RodionovaEmail author
  • V. N. Petushkov


The results of a comparative study of the luciferin–luciferase systems of seven species of bioluminescing oligochaetes—Henlea petushkovi, Henlea rodionovae, Fridericia heliota (Enchytraeidae), Microscolex phosphoreus (Acanthodrilidae), Pontodrilus litoralis (Megascolecidae), Eisenia lucens, and Avelona ligra (Lumbricidae)—are presented.



We are grateful to Dr. Yuichi Oba (Chubu University, Japan), Dr. Peter Taborsky (Masaryk University, Czech Republic), and Dr. G. Kratassiouk (Université Paris Sud, France), who kindly provided the specimens of worms.


This work was supported by the State Assignment for Basic Research of the Russian Academy of Sciences (project no. 0356-2019-0019) and the Russian Foundation for Basic Research (project no. 19-04-00348-a).


Conflict of interests. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.


  1. 1.
    Wampler, J.E. and Jamieson, B.G.M., Earthworm bioluminescence: comparative physiology and biochemistry, Comp. Biochem. Physiol. B, 1980, vol. 66, pp. 43–50.CrossRefGoogle Scholar
  2. 2.
    Wampler, J.E. and Jamieson, B.G.M., Cell bound bioluminescence from Pontodrilus bermudensis, and its similarities to other earthworm bioluminescence, Comp. Biochem. Physiol. A, 1986, vol. 84, no. 1, pp. 81–87.CrossRefGoogle Scholar
  3. 3.
    Bellisario, R., Spencer, T.E., and Cormier, M.J., Isolation and properties of luciferase, a non-heme peroxidase from the bioluminescent earthworm, Diplocardia longa, Biochemistry, 1972, vol. 11, no. 12, pp. 2256–2266.CrossRefPubMedGoogle Scholar
  4. 4.
    Petushkov, V.N. and Rodionova, N.S., New types of luminescent systems of soil enchytraeids (Annelida: Clitellata: Oligochaeta: Enchytraeidae), Dokl. Biochem. Biophys., 2005, vol. 401, pp. 115–118.CrossRefPubMedGoogle Scholar
  5. 5.
    Petushkov, V.N., Dubinnyi, M.A., Tsarkova, A.S., Rodionova, N.S., Baranov, M.S., Kublitski, V.S., Shimomura, O., and Yampolsky, I.V., A novel type of luciferin from the siberian luminous earthworm Fridericia heliota: structure elucidation by spectral studies and total synthesis, Angew. Chem., Int. Ed. Engl., 2014, vol. 53, pp. 5566–5568.CrossRefGoogle Scholar
  6. 6.
    Dubinnyi, M.A., Kaskova, Z.M., Rodionova, N.S., Baranov, M.S., Gorokhovatsky, A.Y., Kotlobay, A., Solntsev, K.M., Tsarkova, A.S., Petushkov, V.N., and Yampolsky, I.V., Novel mechanism of bioluminescence: oxidative decarboxylation of a moiety adjacent to the light emitter of Fridericia luciferin, Angew. Chem., Int. Ed. Engl., 2015, vol. 54, no. 24, pp. 7065–7067.CrossRefGoogle Scholar
  7. 7.
    Rota, E., Martinsson, S., and Erseus, C., Two new bioluminescent Henlea from Siberia and lack of molecular support for Hepatogaster (Annelida, Clitellata, Enchytraeidae), Org. Divers. Evol., 2018, vol. 18, no. 3, pp. 291–312.CrossRefGoogle Scholar
  8. 8.
    Petushkov, V.N., Rodionova, N.S., Purtov, K.V., and Bondar, V.S., The luminescence system of soil enchytraeids, Henlea sp., (Annelida: Clitellata: Oligochaeta: Enchytraeidae), Dokl. Biol. Sci., 2002, vol. 385, pp. 310–312.CrossRefPubMedGoogle Scholar
  9. 9.
    Petushkov, V.N. and Rodionova, N.S., Low-Molecular-Weight Components of Luminescent Reaction of the Siberian Enchytraeid Henlea sp., Dokl. Biochem. Biophys., 2018, vol. 481, pp. 212–216.CrossRefPubMedGoogle Scholar
  10. 10.
    Pes, O., Midlik, A., Schlaghamersky, J., Zitnan, M., and Taborsky, P., A study on bioluminescence and photoluminescence in the earthworm Eisenia lucens, Photochem. Photobiol. Sci, 2016, vol. 15, no. 2, pp. 175–180.CrossRefPubMedGoogle Scholar
  11. 11.
    Rota, E., Martinsson, S., Erseus, C., Petushkov, V.N., Rodionova, N.S., and Omodeo, P., Green light to an integrative view of Microscolex phosphoreus (Duges, 1837) (Annelida: Clitellata: Acanthodrilidae), Zootaxa, 2018, vol. 4496, no. 1, pp. 175–189.CrossRefPubMedGoogle Scholar
  12. 12.
    Wampler, J.E., The bioluminescence system of Microscolex phosphoreus and its similarities to those of other bioluminescent earthworms (Oligochaeta), Comp. Biochem. Physiol. A, 1982, vol. 71, pp. 599–604.CrossRefGoogle Scholar
  13. 13.
    Kin, I. and Oba, Y., A protein related to the luminescence of the earthworm, Microscolex phosphoreus, Abstract, in 20th International Symposium for Bioluminescence and Chemiluminescence, May 2018, Nantes, France, 2018.Google Scholar

Copyright information

© Pleiades Publishing, Ltd. 2019

Authors and Affiliations

  1. 1.Institute of Biophysics, Krasnoyarsk Research Center, Siberian Branch, Russian Academy of SciencesKrasnoyarskRussia

Personalised recommendations