Advertisement

Doklady Biochemistry and Biophysics

, Volume 483, Issue 1, pp 363–368 | Cite as

Ectopic Expression of the Homeotic MADS-Box Gene HAM31 (Helianthus annuus L.) in Transgenic Plants Nicotiana tabacum L. Affects the Gynoecium Identity

  • A. V. ShchennikovaEmail author
  • O. A. Shulga
  • K. G. Skryabin
Biochemistry, Biophysics, and Molecular Biology
  • 13 Downloads

Abstract

The structure of the MADS-box gene HAM31 of the sunflower (Helianthus annuus) was characterized. It is shown that the product of this gene is an ortholog of the B-class MADS transcription factor PISTILLATA (Arabidopsis thaliana). Two types of transgenic tobacco plants (Nicotiana tabacum) with the constitutive expression of the HAM31 gene in the sense and antisense orientation were obtained. The 35S::HAM31s plants formed flowers with an altered gynoecium identity, whereas 35S::HAM31as plants did not differ from the control.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Von Goethe, J.W., The Metamorphosis of Plants, Cambridge, Massachusetts: the MIT Press, 2009, p. 156, paragraph 120, p. 102.Google Scholar
  2. 2.
    Theißen, G., Melzer, R., and Rümpler, F., Development, 2016, vol. 143, no. 18, pp. 3259–3271.CrossRefGoogle Scholar
  3. 3.
    Ronse De Craene, L.P., Ann. Bot., 2007, vol. 100, no. 3, pp. 621–630.CrossRefGoogle Scholar
  4. 4.
    Jack, T., Brockman, L.L., and Meyerowitz, E.M., Cell, 1992, vol. 68, pp. 683–697.CrossRefGoogle Scholar
  5. 5.
    Goto, K. and Meyerowitz, E.M., Genes Dev., 1994, vol. 8, pp. 1548–1560.CrossRefGoogle Scholar
  6. 6.
    Sommer, H., Beltran, J.P., Huijser, P., Pape, H., Lonnig, W.E., Saedler, H., and Schwarz-Sommer, Z., EMBO J., 1990, vol. 9, pp. 605–613.CrossRefGoogle Scholar
  7. 7.
    Trobner, W., Ramirez, L., Motte, P., Hue, I., Huijser, P., Lonnig, W.E., Saedler, H., Sommer, H., and Schwarz-Sommer, Z., EMBO J., 1992, vol. 11, pp. 4693–4704.CrossRefGoogle Scholar
  8. 8.
    Stellari, G.M., Jaramillo, M.A., and Kramer, E.M., Mol. Biol. Evol., 2004, vol. 21, pp. 506–519.CrossRefGoogle Scholar
  9. 9.
    Knowles, P.F., Morphology and anatomy, in Sunflower Science and Technology, Madison, WI: American Society of Agronomy, Crop Science of America, Soil Science Society of America, Inc., 1978, pp. 55–87.Google Scholar
  10. 10.
    Shulga, O.A., Shchennikova, A.V., Angenent, G.C., and Skryabin, K.G., Russ. J. Dev. Biol., 2008, vol. 39, no. 1, pp. 2–5.CrossRefGoogle Scholar
  11. 11.
    Tamura, K., Stecher, G., Peterson, D., Filipski, A., and Kumar, S., Mol. Biol. Evol., 2013, vol. 30, no. 12, pp. 2725–2729.CrossRefGoogle Scholar
  12. 12.
    Mao, W.T., Hsu, H.F., Hsu, W.H., Li, J.Y., Lee, Y.I., and Yang, C.H., Plant Cell Physiol., 2015, vol. 56, no. 11, pp. 2079–2099.Google Scholar
  13. 13.
    Goloveshkina, E.N., Shchennikova, A.V., Kamionskaya, A.M., Skryabin, K.G., and Shulga, O.A., Plant Cell Tiss. Organ Cult., 2012, vol. 109, no. 1, pp. 61–71.CrossRefGoogle Scholar
  14. 14.
    Davies, B., Di Rosa, A., Eneva, T., Saedler, H., and Sommer, H., Plant J., 1996, vol. 10, no. 4, pp. 663–677.CrossRefGoogle Scholar
  15. 15.
    Bowman, J.L., Smyth, D.R., and Meyerowitz, E.M., Development, 1991, vol. 112, no. 1, pp. 1–20.Google Scholar

Copyright information

© Pleiades Publishing, Inc. 2018

Authors and Affiliations

  • A. V. Shchennikova
    • 1
    Email author
  • O. A. Shulga
    • 1
    • 2
  • K. G. Skryabin
    • 1
  1. 1.Institute of Bioengineering, Research Center of BiotechnologyRussian Academy of SciencesMoscowRussia
  2. 2.All-Russia Research Institute of Agricultural BiotechnologyMoscowRussia

Personalised recommendations