Skip to main content
SpringerLink
Log in

Cloning and Functional Analysis of SaCLCc1, a Gene Belonging to the Chloride Channel Family (CLC), from the Halophyte Suaeda altissima (L.) Pall.

  • Biochemistry, Biophysics, and Molecular Biology
  • Published:
Doklady Biochemistry and Biophysics Aims and scope Submit manuscript

Abstract

One of the genes of the CLC (Chloride Channel) family, SaCLCc1, from the halophyte Suaeda altissima (L.) Pall. was cloned. To investigate the function of SaCLCc1, it was expressed in the S. cerevisiae deletion mutant Δgef1::LEU2 for the only gene of the CLC family in this organism. The growth of the transformed SaCLCc1-expressing mutant Δgef1 was restored when cells were grown in Fe2+-deficient YPEG medium, in minimal synthetic media SD and SR (pH 7.0), and in rich YPD medium containing Mn2+. The complementation of the Δgef1 mutant phenotype with the SaClCc1 gene indicates the involvement of the SaClCc1 protein in the transport of Cl– ions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Barbier-Brygoo, H., De Angeli, A., Filleur, S., Frachisse, J.-M., Cambale, F., Thomine, S., and Wege, S., Ann. Rev. Plant Biol., 2011, vol. 62, pp. 25–51

    Article  CAS  Google Scholar 

  2. Jossier, M., Kroniewicz, L., Dalmas, F., Le Thiec, D., Ephritikhine, G., Thomine, S., Barbier-Brygoo, H., Vavasseur, A., Filleur, S., and Leonhardt, N., Plant J., 2010, vol. 64, no. 4, pp. 563–576

    Article  PubMed  CAS  Google Scholar 

  3. Shuvalov, A.V., Orlova, J.V., Khalilova, L.A., Myasoedov, N.A., Andreev, I.M., Belyaev, D.V., and Balnokin, Y.V., Russ. J. Plant Physiol., 2015, vol. 62, no. 1, pp. 45–46

    Article  CAS  Google Scholar 

  4. Lopez-Rodriguez, A., Trejo, A.C., Coyne, L., Halliwell, R.F., Miledi, R., and Martinez-Torres, A., FEMS Yeast Res., 2007, vol. 7, pp. 1218–1229

    Article  PubMed  CAS  Google Scholar 

  5. Gaxiola, R.A., Yuan, D.S., Klausner, R.D., and Fink, G.R., Proc. Natl. Acad. Sci. U. S. A., 1998, vol. 95, pp. 4046–4050

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  6. Robinson, S.P. and Downton, W.J.S., Aust. J. Plant Physiol., 1985, vol. 12, pp. 471–479

    Article  CAS  Google Scholar 

  7. Diray-Arce, J., Clement, M., Gul, B., Khan, M.A., and Nielsen, B.L., BMC Genomics, 2015, vol. 16, no. 353.

    Google Scholar 

  8. Andrews, S., 2010. https://doi.org/www.bioinformatics.bbsrc.ac.uk/projects/fastqc/.

  9. Bolger, A.M., Lohse, M., and Usadel, B., Bioinformatics, 2014, btu170.

    Google Scholar 

  10. Haas, B.J., Papanicolaou, A., Yassour, M., Grabherr, M., Blood, P.D., et al., Nat. Protoc., 2013, vol. 8, pp. 1494–1512.

    Article  PubMed  CAS  Google Scholar 

  11. Bairoch, A., Boeckmann, B., Ferro, S., and Gasteiger, E., Brief Bioinform., 2004, vol. 5, pp. 39–55

    Article  PubMed  CAS  Google Scholar 

  12. Buchfink, B., Xie, C., and Huson, D.H., Nat. Methods, 2015, vol. 12, pp. 59–60

    Article  PubMed  CAS  Google Scholar 

  13. Gibson, D.G., Young, L., Chuang, R.-Y., Venter, J.C., Hutchison, C.A., III., and Smith, H.O., Nat. Methods, 2009, vol. 6, pp. 343–345

    Article  PubMed  CAS  Google Scholar 

  14. Eldarov, M.A., Baranov, M.V., Dumina, M.V., Shgun, A.A., Andreeva, N.A., Trilisenko, L.V., Kulakovskaya, T.V., Ryasanova, L.P., and Kulaev, I.S., Biochemistry (Moscow), 2013, vol. 78, no. 8, pp. 1201–1209

    Article  CAS  Google Scholar 

  15. Zifarelli, G. and Pusch, M., FEBS Lett., 2010, vol. 584, pp. 2122–2127

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, 127276, Russia

    O. I. Nedelyaeva, A. V. Shuvalov, O. V. Mayorova, L. G. Popova, Yu. V. Balnokin & I. V. Karpichev

  2. Dobrzhansky Center for Genomic Bioinformatics, St. Petersburg State University, St. Petersburg, 199034, Russia

    A. A. Yurchenko

  3. Moscow State University, Moscow, 119234, Russia

    Yu. V. Balnokin

Authors
  1. O. I. Nedelyaeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. V. Shuvalov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. O. V. Mayorova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. A. Yurchenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. L. G. Popova
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yu. V. Balnokin
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. I. V. Karpichev
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to O. I. Nedelyaeva.

Additional information

Original Russian Text © O.I. Nedelyaeva, A.V. Shuvalov, O.V. Mayorova, A.A. Yurchenko, L.G. Popova, Yu.V. Balnokin, I.V. Karpichev, 2018, published in Doklady Akademii Nauk, 2018, Vol. 481, No. 1.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Nedelyaeva, O.I., Shuvalov, A.V., Mayorova, O.V. et al. Cloning and Functional Analysis of SaCLCc1, a Gene Belonging to the Chloride Channel Family (CLC), from the Halophyte Suaeda altissima (L.) Pall.. Dokl Biochem Biophys 481, 186–189 (2018). https://doi.org/10.1134/S1607672918040026

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1607672918040026

Search

Navigation