Abstract
Fluorescent derivatives are widely used to study the structure and functions of proteins. Quantum dots (QDs), fluorescent semiconductor nanocrystals, have a high quantum yield and are much more resistant to bleaching compared to organic dyes. Conjugates of α-neurotoxins with QDs were used for visualization of human α7 acetylcholine receptors heterologously expressed in GH4C1 pituitary adenoma cells. Specific staining of cells by the conjugated toxins was observed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Tsetlin, V.I., Karlsson, E., Utkin, Yu.N., Pluzhnikov, K.A., Arseniev, A.S., Surin, A.M., Kondakov, V.V., Bystrov, V.F., Ivanov, V.T., and Ovchinnikov, Yu.A., Toxicon, 1982, vol. 20, no. 1, pp. 83–93.
Johnson, D.A., Cushman, R., and Malekzadeh, R., J. Biol. Chem., 1990, vol. 265, no. 13, pp. 7360–7368.
Shelukhina, I.V., Kryukova, E.V., Lips, K.S., Tsetlin, V.I., and Kummer, W., J. Neurochem., 2009, vol. 109, no. 4, pp. 1087–1095.
Burli, T., Baer, K., Ewers, H., Sidler, C., Fuhrer, C., and Fritschy, J.M., PLoS One, 2010, vol. 5, no. 7, p. e11507.
Gomez-Varela, D. and Berg, D.K., J. Neurosci., 2013, vol. 33, no. 43, pp. 17062–17071.
Utkin, Y.N., Cherepakhin, I.Y., Kryukova, E.V., Shelukhina, I.V., Makarova, Y.V., Kasheverov, I.E., Mukherjee, A.K., Gusev, A.A., and Kuznetsov, D.V., Nano Hybrids Composites, 2017, vol. 13, pp. 3–8.
Utkin, Y.N., Weise, C., Kasheverov, I.E., Andreeva, T.V., Kryukova, E.V., Zhmak, M.N., Starkov, V.G., Hoang, N.A., Bertrand, D., Ramerstorfer, J., Sieghart, W., Thompson, A.J., Lummis, S.C., and Tsetlin, V.I., J. Biol. Chem., 2012, vol. 287, no. 32, pp. 27079–27086.
Bencherif, M., Narla, S.T., and Stachowiak, M.S., CNS Neurol. Disord. Drug. Targets, 2014, vol. 13, no. 5, pp. 836–845.
Zdanowski, R., Krzyzowska, M., Ujazdowska, D., Lewicka, A., and Lewicki, S., Cent. Eur. J. Immunol., 2015, vol. 40, no. 3, pp. 373–379.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © Ya.V. Makarova, I.V. Shelukhina, A.K. Mukherjee, D.V. Kuznetsov, V.I. Tsetlin, Yu.N. Utkin, 2017, published in Doklady Akademii Nauk, 2017, Vol. 475, No. 1, pp. 103–105.
Rights and permissions
About this article
Cite this article
Makarova, Y.V., Shelukhina, I.V., Mukherjee, A.K. et al. Detection of human neuronal α7 nicotinic acetylcholine receptors by conjugates of snake α-neurotoxin with quantum dots. Dokl Biochem Biophys 475, 253–255 (2017). https://doi.org/10.1134/S1607672917040032
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1607672917040032