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The fluorescence lifetime of lipofuscin granule fluorophores contained in the retinal pigment epithelium cells from human cadaver eyes in normal state and in the case of visualized pathology

  • Biochemistry, Biophysics, and Molecular Biology
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Abstract

A comparative analysis of fluorescence lifetime of lipofuscin granule fluorophores contained in the retinal pigment epithelium cells from human cadaver eyes in normal state and in the case of visualized pathology was carried out. Measurements of fluorescence lifetimes of bis-retinoids and their photooxidation and photodegradation products were carried out using the method of counting time-correlated photons. Comparative analysis showed that, in the case of visualized pathology, the contribution of photooxidation and photodegradation products of bis-retinoids to the total fluorescence of the retinal pigment epithelium cell suspension increases in comparison with the norm.

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Authors and Affiliations

  1. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, 119334, Russia

    M. A. Yakovleva, T. B. Feldman, V. A. Kuzmin & M. A. Ostrovsky

  2. Biological Faculty, Moscow State University, Moscow, 119991, Russia

    T. B. Feldman & M. A. Ostrovsky

  3. Fedorov Eye Microsurgery Research and Technology Complex, Ministry of Health of the Russian Federation, Moscow, 127486, Russia

    P. M. Arbukhanova & S. A. Borzenok

Authors
  1. M. A. Yakovleva
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  2. T. B. Feldman
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  3. P. M. Arbukhanova
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  4. S. A. Borzenok
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  5. V. A. Kuzmin
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  6. M. A. Ostrovsky
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Corresponding author

Correspondence to M. A. Yakovleva.

Additional information

Original Russian Text © M.A. Yakovleva, T.B. Feldman, P.M. Arbukhanova, S.A. Borzenok, V.A. Kuzmin, M.A. Ostrovsky, 2017, published in Doklady Akademii Nauk, 2017, Vol. 474, No. 6, pp. 760–765.

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Yakovleva, M.A., Feldman, T.B., Arbukhanova, P.M. et al. The fluorescence lifetime of lipofuscin granule fluorophores contained in the retinal pigment epithelium cells from human cadaver eyes in normal state and in the case of visualized pathology. Dokl Biochem Biophys 474, 239–243 (2017). https://doi.org/10.1134/S1607672917030231

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  • DOI: https://doi.org/10.1134/S1607672917030231

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