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Modification of human serum albumin under induced oxidation

  • Biochemistry, Biophysics, and Molecular Biology
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Abstract

For the first time, by using the complex of physicochemical methods (mass-spectrometry, differential scanning calorimetry, spectrofluorimetry, method of spectral and fluorescent probes, dynamic light scattering, and UV spectrophotometry), the oxidation-mediated modification of chemical and spatial structure of albumin has been studied. All albumin structural regions are subjected to oxidation, methionine and aromatic amino acids primarily involved in oxidation. The albumin melting shows a decrease in thermal stabilization of the structure and changing of aggregation upon oxidation. The change in physical and chemical properties of albumin under different quantity of the oxidizer has been analyzed.

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Authors and Affiliations

  1. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, 119334, Russia

    M. G. Gorobets, L. A. Wasserman, A. D. Vasilyeva, A. V. Bychkova, P. G. Pronkin, A. E. Bugrova, M. I. Indeykina, N. G. Shilkina, M. L. Konstantinova, A. S. Kononikhin, E. N. Nikolaev & M. A. Rosenfeld

  2. Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Moscow, Russia

    A. S. Kononikhin

  3. Moscow Institute of Physics and Technology, Dolgoprudny, Moscow oblast, Russia

    M. I. Indeykina, A. S. Kononikhin & E. N. Nikolaev

  4. Skolkovo Institute of Science and Technology, Skolkovo, 143025, Russia

    E. N. Nikolaev

Authors
  1. M. G. Gorobets
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  2. L. A. Wasserman
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  3. A. D. Vasilyeva
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  4. A. V. Bychkova
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  5. P. G. Pronkin
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  6. A. E. Bugrova
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  7. M. I. Indeykina
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  8. N. G. Shilkina
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  9. M. L. Konstantinova
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  10. A. S. Kononikhin
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  11. E. N. Nikolaev
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  12. M. A. Rosenfeld
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Corresponding author

Correspondence to M. G. Gorobets.

Additional information

Original Russian Text © M.G. Gorobets, L.A. Wasserman, A.D. Vasilyeva, A.V. Bychkova, P.G. Pronkin, A.E. Bugrova, M.I. Indeykina, N.G. Shilkina, M.L. Konstantinova, A.S. Kononikhin, E.N. Nikolaev, M.A. Rosenfeld, 2017, published in Doklady Akademii Nauk, 2017, Vol. 474, No. 6, pp. 751–755.

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Gorobets, M.G., Wasserman, L.A., Vasilyeva, A.D. et al. Modification of human serum albumin under induced oxidation. Dokl Biochem Biophys 474, 231–235 (2017). https://doi.org/10.1134/S1607672917030218

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  • DOI: https://doi.org/10.1134/S1607672917030218

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