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Flavoprotein miniSOG BRET-induced cytotoxicity depends on its intracellular localization

  • Biochemistry, Biophysics, and Molecular Biology
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Abstract

It is proposed to use the bioluminescent resonance energy transfer to solve the problem of creating the internal light sources in photodynamic therapy of cancer. Energy donor in the developed system is the oxidized form of the luciferase NanoLuc substrate furimamide, and acceptor is the phototoxic fluorescent protein miniSOG. It is shown that, in the proposed system, the photoinduced cytotoxicity of flavoprotein miniSOG in vitro depends on the intracellular localization, and the cytotoxic effect is 48% for the cytoplasmic localization of the fusion protein, 65% for the mitochondrial localization, and 69% for the membrane localization. The obtained data indicate that, for maximization of the photodynamic effect in vivo, it is appropriate to use the NanoLuc–miniSOG fusion protein in the membrane localization.

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Authors and Affiliations

  1. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia

    E. I. Shramova, G. M. Proshkina, S. M. Deyev & R. V. Petrov

  2. Lobachevsky Nizhny Novgorod State University, Nizhny Novgorod, 603950, Russia

    S. M. Deyev

Authors
  1. E. I. Shramova
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  2. G. M. Proshkina
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  3. S. M. Deyev
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  4. R. V. Petrov
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Corresponding author

Correspondence to E. I. Shramova.

Additional information

Original Russian Text © E.I. Shramova, G.M. Proshkina, S.M. Deyev, R.V. Petrov, 2017, published in Doklady Akademii Nauk, 2017, Vol. 474, No. 5, pp. 633–636.

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Shramova, E.I., Proshkina, G.M., Deyev, S.M. et al. Flavoprotein miniSOG BRET-induced cytotoxicity depends on its intracellular localization. Dokl Biochem Biophys 474, 228–230 (2017). https://doi.org/10.1134/S160767291703019X

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  • DOI: https://doi.org/10.1134/S160767291703019X

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