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Stereoselective biotransformation of phenylglycine nitrile by heterogeneous biocatalyst based on immobilized bacterial cells and enzyme preparation

  • Biochemistry, Biophysics, and Molecular Biology
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Abstract

We studied the effect of a heterogeneous environment on the stereoselectivity of transformation of racemic phenylglycine nitrile. Immobilized biocatalysts were prepared by adhesion of Pseudomonas fluorescens C2 cells on carbon-containing supports and covalent crosslinking of nitrile hydratase and amidase of Rhodococcus rhodochrous 4–1 to activated chitosan as well as by the method of cross-linked aggregates. At a reaction duration of 20 h, the ratio of phenylglycine stereoisomers changes depending on the presence of support in medium. The highest optical purity of the product (enantiomeric excess of L-phenylglycine solution, 98%) is achieved when enzyme aggregates of nitrile hydratase and amidase cross-linked with 0.1% glutaraldehyde are used as a biocatalyst.

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Authors and Affiliations

  1. Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, Perm, 614081, Russia

    Yu. G. Maksimova, A. N. Gorbunova & V. A. Demakov

  2. Perm State National Research University, Perm, 614600, Russia

    Yu. G. Maksimova, A. N. Gorbunova & V. A. Demakov

Authors
  1. Yu. G. Maksimova
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  2. A. N. Gorbunova
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  3. V. A. Demakov
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Corresponding author

Correspondence to Yu. G. Maksimova.

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Original Russian Text © Yu.G. Maksimova, A.N. Gorbunova, V.A. Demakov, 2017, published in Doklady Akademii Nauk, 2017, Vol. 474, No. 2, pp. 248–250.

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Maksimova, Y.G., Gorbunova, A.N. & Demakov, V.A. Stereoselective biotransformation of phenylglycine nitrile by heterogeneous biocatalyst based on immobilized bacterial cells and enzyme preparation. Dokl Biochem Biophys 474, 183–185 (2017). https://doi.org/10.1134/S1607672917030139

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  • DOI: https://doi.org/10.1134/S1607672917030139

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