Abstract
Using Fura-2AM microfluorimetry, we have shown for the first time that preincubation of macrophages with methyl-β-cyclodextrin, inducing cholesterol extraction from membranes and raft disruption, leads to significant inhibition of thapsigargin-induced store-dependent Ca2+ entry in rat peritoneal macrophages. In contrast, macrophage treatment with methyl-β-cyclodextrin after Ca2+ entry mechanisms were activated by store depletion by thapsigargin application leads to potentiation of subsequent store-dependent Ca2+ entry. The results suggest that intact lipid rafts are necessary for the activation but not the maintenance of store-dependent Ca2+ entry in macrophages.
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Original Russian Text © Z.I. Krutetskaya, L.S. Milenina, A.A. Naumova, S.N. Butov, V.G. Antonov, A.D. Nozdrachev, 2017, published in Doklady Akademii Nauk, 2017, Vol. 473, No. 2, pp. 222–224.
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Krutetskaya, Z.I., Milenina, L.S., Naumova, A.A. et al. Methyl-β-cyclodextrin modulates thapsigargin-induced store-dependent Ca2+ entry in macrophages. Dokl Biochem Biophys 473, 88–90 (2017). https://doi.org/10.1134/S1607672917020028
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DOI: https://doi.org/10.1134/S1607672917020028