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Mathematics analysis of polymerase chain reaction kinetic curves

  • Biochemistry, Biophysics and Molecular Biology
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Abstract

The paper reviews different approaches to the mathematical analysis of polymerase chain reaction (PCR) kinetic curves. The basic principles of PCR mathematical analysis are presented. Approximation of PCR kinetic curves and PCR efficiency curves by various functions is described. Several PCR models based on chemical kinetics equations are suggested. Decision criteria for an optimal function to describe PCR efficiency are proposed.

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Authors and Affiliations

  1. JSC Syntol, ul. Timiryazevskaya 42, Moscow, 127550, Russia

    D. G. Sochivko

  2. Institute for Analytical Instrumentation, Russian Academy of Sciences, Rizhskii pr. 26, St. Petersburg, 190103, Russia

    A. A. Fedorov & V. E. Kurochkin

  3. All-Russia Research Institute of Agricultural Biotechnology, ul. Timiryazevskaya 42, Moscow, 127550, Russia

    D. A. Varlamov

  4. National Research Center, Institute of Immunology, Federal Biomedical Agency, Kashirskoe sh. 24, Moscow, 115478, Russia

    R. V. Petrov

Authors
  1. D. G. Sochivko
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  2. A. A. Fedorov
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  3. D. A. Varlamov
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  4. V. E. Kurochkin
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  5. R. V. Petrov
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Corresponding author

Correspondence to A. A. Fedorov.

Additional information

Original Russian Text © D.G. Sochivko, A.A. Fedorov, D.A. Varlamov, V.E. Kurochkin, R.V. Petrov, 2016, published in Doklady Akademii Nauk, 2016, Vol. 466, No. 1, pp. 109–113.

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Sochivko, D.G., Fedorov, A.A., Varlamov, D.A. et al. Mathematics analysis of polymerase chain reaction kinetic curves. Dokl Biochem Biophys 466, 13–16 (2016). https://doi.org/10.1134/S160767291601004X

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  • DOI: https://doi.org/10.1134/S160767291601004X

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