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WNT-associated gene expression in human mesenchymal stromal cells under hypoxic stress

  • Biochemistry, Biophysics and Molecular Biology
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Abstract

The effect of short-term stress on the expression of 84 Wnt-related genes in mesenchymal stromal cells (MSCs) was analyzed. In MSCs permanently expanded at 5% O2, Dkk1, RHOA, CSNK1A1, CSNK2A1, GSK3β, and PORCN genes were most highly expressed. FZD1, FZD3, SFRP1, and SFRP4 genes were up-regulated after short-term hypoxic stress (0.1% O2), whereas the expression of LEF-1 and RUVBL1 genes was significantly inhibited (down-regulated). The data suggest that Wnt signal transduction was suppressed under hypoxic stress, whereas the noncanonical Wnt signaling prevailed under the “physiological hypoxia” (5% O2).

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Authors and Affiliations

  1. Institute of Biomedical Problems, Russian Academy of Sciences, Khoroshevskoe shosse 76-a, Moscow, 123007, Russia

    O. O. Udartseva, E. R. Andreeva & L. B. Buravkova

Authors
  1. O. O. Udartseva
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  2. E. R. Andreeva
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  3. L. B. Buravkova
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Correspondence to O. O. Udartseva.

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Original Russian Text © O.O. Udartseva, E.R. Andreeva, L.B. Buravkova, 2015, published in Doklady Akademii Nauk, 2015, Vol. 465, No. 1, pp. 111–115.

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Udartseva, O.O., Andreeva, E.R. & Buravkova, L.B. WNT-associated gene expression in human mesenchymal stromal cells under hypoxic stress. Dokl Biochem Biophys 465, 354–357 (2015). https://doi.org/10.1134/S1607672915060046

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  • DOI: https://doi.org/10.1134/S1607672915060046

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