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Electrosynthesis and binding properties of molecularly imprinted poly-o-phenylenediamine as artificial antibodies for electroanalysis of myoglobin

  • Biochemistry, Biophysics and Molecular Biology
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Abstract

Molecularly imprinted poly-o-phenylenediamine with template myoglobin molecules (i.e., polymeric antibodies to myoglobin, molecularly imprinted polymer, MIP) was synthesized via electropolymerization. Electropolymerization, washing, and the interaction of the polymeric antibodies with myoglobin was examined by square wave voltammetry and microgravimetry. The analysis of myoglobin was carried out through direct electrochemical detection of the reduction peak of Fe3+ of the hemeprotein on screen-printed graphite electrodes modified by the MIP. According to the electrochemical analysis, MIP surfaces demonstrated remarkably higher ability to bind the protein compared to that of surfaces prepared by the same route under the same conditions but in the absence of myoglobin (surfaces of the non-imprinted polymer, NIP). The imprinting factor I max(MIP)/I max(NIP) was found to be 2–4. The equilibrium dissociation constant K d of the interaction of myoglobin with MIP electrodes was evaluated as (2.4 ± 0.5) × 10–8 M. The lower detection limit of myoglobin by a MIP sensor was determined as 0.5 × 10–9 M, the range of detectable concentrations being 10–9–10–5 M.

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References

  1. Petrovskaya, L.E., Shingarova, L.N., Dolgikh, D.A., and Kirpichnikov, M.P., Bioorg. Khim., 2011, vol. 37, pp. 517–526.

    CAS  Google Scholar 

  2. Luo, J., Jiang, S., and Liu, X., Sens. Actuators B, 2014, vol. 203, pp. 782–789.

    Article  CAS  Google Scholar 

  3. Gendrikson, O.D., Zherdev, A.V., and Dzantiev, B.B., Usp. Biol. Khim., 2006, vol. 46, pp. 149–192.

    Google Scholar 

  4. Li, S., Cao, S., Whitcombe, M., and Piletsky, S., Prog. Polym. Sci., 2014, vol. 39, pp. 145–163.

    Article  CAS  Google Scholar 

  5. Cai, D., Ren, L., Zhao, H., Xu, C., Zhang, L., Yu, Y., Wang, H., Lan, Y., Roberts, M.F., Chuang, J.H., Naughton, M.J., Ren, Z., and Chiles, T.C., Nature Nanotechnol., 2010, vol. 5, pp. 597–601.

    Article  CAS  Google Scholar 

  6. Tiwari, A., Deshpandea, S.R., Kobayashi, H., and Turner, A.P.F., Biosens. Bioelectron., 2012, vol. 35, pp. 224–229.

    Article  CAS  PubMed  Google Scholar 

  7. Lv, Y., Tan, T., and Svec, F., Biotechnol. Adv., 2013, vol. 31, pp. 1172–1186.

    Article  CAS  PubMed  Google Scholar 

  8. Sharma, P., Iskierko, Z., Pietrzyk-Le, A., D’Souza, F., and Kutner, W., Electrochem. Commun., 2015, vol. 50, pp. 81–87.

    Article  CAS  Google Scholar 

  9. Polyakov, M.V., Zh. Fiz. Khim., 1931, no. 2, pp. 799–805.

    Google Scholar 

  10. Qureshi, A., Gurbuz, Y., and Niazi, J., Sens. Actuators B, 2012, vols. 171–172, p. 62–76.

    Article  Google Scholar 

  11. McDonnell, B., Hearty, S., Leonard, P., and O’Kennedy, R., Clin. Biochem., 2009, vol. 42, pp. 549–561.

    Article  CAS  PubMed  Google Scholar 

  12. Karimian, N., Turner, A., and Tiwari, A., Biosens. Bioelectron., 2014, vol. 59, pp. 160–165.

    Article  CAS  PubMed  Google Scholar 

  13. Sigolaeva, L.V., Günter, U., Pergushov, D.V., Gladyr, S.Yu., Kurochkin, I.N., and Schacher, F.H., Macromol. Biosci., 2014, vol. 14, no. 7, pp. 1039–1051.

    Article  CAS  PubMed  Google Scholar 

  14. Shumyantseva, V., Bulko, T., Vagin, M., Suprun, E., and Archakov, A., Biochemistry (Moscow) Suppl. Ser. B. Biomed. Chem., 2010, vol. 4, pp. 237–242.

    Article  Google Scholar 

  15. Suprun, E., Bulko, T., Lisitsa, A., Gnedenko, O., Ivanov, A., Shumyantseva, V., and Archakov, A., Biosens. Bioelectron., 2010, vol. 25, pp. 1694–1698.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Orekhovich Biomedical Chemistry Institute, Moscow, 119121, Russia

    V. V. Shumyantseva, T. V. Bulko, L. V. Sigolaeva, A. V. Kuzikov & A. I. Archakov

  2. LLC IBMC–EcoBioFarm, Moscow, 119121, Russia

    V. V. Shumyantseva, T. V. Bulko & A. V. Kuzikov

  3. Department of Chemistry, Lomonosov Moscow State University, Moscow, 119991, Russia

    L. V. Sigolaeva

  4. Pirogov Russian National Medical University, Moscow, 117997, Russia

    M. A. Shatskaya

Authors
  1. V. V. Shumyantseva
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  2. T. V. Bulko
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  3. L. V. Sigolaeva
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  4. A. V. Kuzikov
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  5. M. A. Shatskaya
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  6. A. I. Archakov
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Corresponding author

Correspondence to V. V. Shumyantseva.

Additional information

Published in Russian in Doklady Akademii Nauk, 2015, Vol. 464, No. 1, pp. 111–114.

The article was translated by the authors.

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Shumyantseva, V.V., Bulko, T.V., Sigolaeva, L.V. et al. Electrosynthesis and binding properties of molecularly imprinted poly-o-phenylenediamine as artificial antibodies for electroanalysis of myoglobin. Dokl Biochem Biophys 464, 275–278 (2015). https://doi.org/10.1134/S1607672915050038

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  • DOI: https://doi.org/10.1134/S1607672915050038

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