Abstract
For the first time, the presence of a new alternative promoter in the gene of the oct-1 transcription factor from which a previously unknown mRNA isoform Oct-1X, with 5′-terminus different from the previously described isoforms, was demonstrated. The nucleotide sequence of the Oct-1X cDNA was determined and the presence of a long open reading frame which starts with the first ATG codon of the second exon was demonstrated. A protein shortened on its N-terminus is assumed to be the product of a new mRNA isoform. Expression of the new isoform was studied in various human cell lines; the results indicate the ubiquitous nature of the Oct-1X expression. Therefore, in the work, the third alternative promoter of human oct-1 gene (earlier, a ubiquitous promoter U and an inducible tissue-specific promoter L were described) contributing to the fine regulation of mRNA isoform synthesis and formation of structural and functional diversity of Oct-1 protein isoforms in cells was revealed.
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Original Russian Text © T.N. Portseva, I.D. Krylova, S.G. Georgieva, A.G. Stepchenko, E.V. Pankratova, 2013, published in Doklady Akademii Nauk, 2013, Vol. 449, No. 1, pp. 110–112.
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Portseva, T.N., Krylova, I.D., Georgieva, S.G. et al. New alternative promoter in regulation of the oct-1 human gene transcription. Dokl Biochem Biophys 449, 72–74 (2013). https://doi.org/10.1134/S160767291302004X
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DOI: https://doi.org/10.1134/S160767291302004X