Skip to main content

Structure of Hydrogels and Activity of Proteins Immobilized in Them


The effect of the chemical structure of crosslinking agents on the activity of proteins in hydrogels obtained by the copolymerization of acrylamide, a crosslinking agent, and an unsaturated protein derivative is studied. It is shown that interaction between units of the hydrophobic crosslinking agent which increases with an increase in the length of the aliphatic region between the double bonds of the crosslinking agent leads to an additional “hydrophobic crosslinking” of macromolecules and causes a decrease in the activity of the immobilized protein.

This is a preview of subscription content, access via your institution.


  1. 1

    P. Angsantikul and A. Mitragotri, Trends Pharmacol. Sci. 41, 681 (2020).

    CAS  Article  Google Scholar 

  2. 2

    T. Wang, G. Qu, C. Wang, Y. Cheng, J. Shang, J. Zheng, Z. Feng, Q. Chen, and N. He, Langmuir 35, 13999 (2019).

    CAS  Article  Google Scholar 

  3. 3

    V. E. Diederich, P. Studer, A. Kern, M. Lattuada, G. Storti, R. I. Sharma, J. G. Snedeker, and M. Morbidelli, Biotechnol. Bioeng. 110, 1508 (2013).

    CAS  Article  Google Scholar 

  4. 4

    K. Nalampang, R. Panjakha, R. Molloy, and B. J. Tighe, J. Biomater. Sci., Polym. Ed. 24, 1291 (2013).

    CAS  Article  Google Scholar 

  5. 5

    C. A. Schoener, H. N. Hutson, and N. A. Peppas, J. Biomed. Mater. Res 101, 2229 (2013).

    Article  Google Scholar 

  6. 6

    L. I. Valuev, I. L. Valuev, L. V. Vanchugova, and I. L. Obydennova, Polym. Sci., Ser. B 57, 456 (2015).

    CAS  Google Scholar 

  7. 7

    L. V. Vanchugova, L. I. Valuev, I. L. Valuev, and Yu. A. Talyzenko, Polym/ Sci., Ser. B 55, 77 (2013).

    CAS  Article  Google Scholar 

  8. 8

    J. Chauvet and R. Acher, J. Biol. Chem. 242, 4274 (1967).

    CAS  Article  Google Scholar 

  9. 9

    V. V. Mosolov, Proteolytic Enzymes (Nauka, Moscow, 1971) [in Russian].

    Google Scholar 

  10. 10

    D. F. Erlanger, N. Kokowski, and W. Cohen, Arch. Biochem. Biophys. 95, 271 (1961).

    CAS  Article  Google Scholar 

  11. 11

    Y. Cohen, O. Ramon, I. J. Kopelman, and S. J. Mizrah, J. Polym. Sci., Part B: Polym. Phys. 30, 1055 (1992).

    CAS  Article  Google Scholar 

  12. 12

    H. Rolfes and R. F. T. Stepto, Makromol. Chem.,Theory Simul. 1, 969 (1992).

    Google Scholar 

  13. 13

    L. I. Valuev, V. V. Chupov, G. A. Sytov, I. M. Shanazarova, L. N. Kislaya, V. A. Sinani, and N. A. Plate, Vysokomol. Soedin., Ser. A 37, 787 (1995).

    CAS  Google Scholar 

  14. 14

    N. A. Plate, L. I. Valuev, V. A. Sinani, and V. V. Chupov, Biotekhnologiya 3, 173 (1987).

    CAS  Google Scholar 

Download references


This work was carried out within the framework of the state task for the Topchiev Institute of Petrochemical Synthesis, Russian Academy of Sciences.

Author information



Corresponding author

Correspondence to I. L. Valuev.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Valuev, I.L., Vanchugova, L.V., Gorshkova, M.Y. et al. Structure of Hydrogels and Activity of Proteins Immobilized in Them. Polym. Sci. Ser. B 63, 404–407 (2021).

Download citation