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H2S-Mediated Dilation of Pial Arteries in Rats of Different Ages: Contribution of KATP and BKCa Channels

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Abstract

Reactions of pial arteries to exogenous hydrogen sulfide exposure and assessment of the contribution of KATP and BKCa channels to H2S-mediated dilation was studied in rats of different ages. Intravital microphotography in Sprague-Dawley rats aged 4 and 18 months was used to study the reactions of pial arteries of various diameters to the exposure of exogenous hydrogen sulfide donor solution—sodium hydrosulfide (NaHS, 30 µM), as well as their change with the preliminary use of potassium channel blockers: KATP (glibenclamide, 10 µM) and BKCa (tetraethyl ammonium, 2 mM). It was found that inhibition of H2S-mediated dilation of pial arteries and increase in constrictor responses to exogenous hydrogen sulfide exposure are taking place in rats with age. Age-related changes in H2S-induced dilatory response of the pial arteries in rats depend on the size of the vessels. With age, there is a decrease in the number of dilations of pial arteries with a diameter of more than 20 µm. At the same time, aging does not affect the dilatation of smaller arteries. These disorders are probably associated with changes in the processes caused by the activation of potassium channels. It was found that aging is accompanied by the increasing of KATP-channels contribution to the implementation of H2S-mediated dilation in pial arteries with diameters less than 40 µm. BKCa-channels contribution to the dilatation decreases with age. In 18-months-old rats, these channels barely participate in H2S-mediated dilation in arteries with diameters more than 20 µm.

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Funding

The source of funding is the state budget. This work was supported by State Program 47 of the State Program “Scientific and Technological Development of the Russian Federation” (2019–2030), topic 0134-2019-0001.

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Authors and Affiliations

  1. Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, Russia

    O. P. Gorshkova & I. B. Sokolova

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  1. O. P. Gorshkova
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  2. I. B. Sokolova
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Contributions

Planning the experiment—O.P.G., collecting and processing data—O.P.G., I.B.S., writing and editing the article—O.P.G., I.B.S..

Corresponding author

Correspondence to O. P. Gorshkova.

Ethics declarations

COMPLIANCE WITH ETHICAL STANDARDS

All procedures performed on animals complied with the ethical standards approved by the legal acts of the Russian Federation, the principles of the Basel Declaration and recommendations of the Commission for the Control over the Keeping and Use of Laboratory Animals at the Pavlov Institute of Physiology of the Russian Academy of Sciences (Minutes no. 12/26 of 12.12.2022). I.P. Pavlov Institute of Physiology of the Russian Academy of Sciences (protocol no. 12/26 of 26.12.2022).

CONFLICT OF INTEREST

The authors declare that they have no conflicts of interest.

Additional information

Translated by A. Dyomina

Russian Text © The Author(s), 2023, published in Rossiiskii Fiziologicheskii Zhurnal imeni I.M. Sechenova, 2023, Vol. 109, No. 8, pp. 1094–1107https://doi.org/10.31857/S0869813923080034.

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Gorshkova, O.P., Sokolova, I.B. H2S-Mediated Dilation of Pial Arteries in Rats of Different Ages: Contribution of KATP and BKCa Channels. J Evol Biochem Phys 59, 1414–1425 (2023). https://doi.org/10.1134/S1234567823040328

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