Abstract
Silver nanoparticles have been fabricated by the method of chemical reduction in solution using different types of stabilizers: an organic low-molecular compound—sodium dioctyl sulfosuccinate (AOT)— and a natural biologically active substance—antimicrobial cationic polypeptide lysozyme. According to studies of the produced hydrosols, the average size of the shell-coated particles is 20–25 nm. The biological activity of the obtained bioconjugates toward Gram-negative (Escherichia coli ML35p, Pseudomonas aeruginosa (clinical isolate)) and Gram-positive (Listeria monocytogenes EGD (АТСС ВАА-679), MRSA ATCC 33591 (Staphylococcus aureus resistant to methicillin)) bacteria has been investigated by the methods of radial diffusion in an agarose gel and serial dilution in a liquid nutritional medium. It has been established that the antimicrobial activity of the bioconjugates depends on the nature of the used stabilizer.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Sharma, V.K., Siskova, K.M., Zboril, R., and Gardea-Torresdey, J.L., Organic-coated silver nanoparticles in biological and environmental conditions: Fate, stability and toxicity, Adv. Colloid Interface Sci., 2014, vol. 204, pp. 15–34.
Le, Y., Mehmood, F., Lee, S., Seifert, S., et al., Increased silver activity for direct propylene epoxidation via subnanometer size effects, Science, 2010, vol. 328, no. 5975, pp. 224–228.
Liu, X.Q., Wang, F., Niazov-Elkan, A., Guo, W.W., and Wilner, O., Probing biocatalytic transformations with luminescent DNA/silver nanoclusters, Nano Lett., 2013, vol. 13, p. 309.
Montenegro, J.-M., Grazu, V., Sukhanova, A., Agarwal, S., de la Fuente, J.V., Nabiev, I., Greiner, A., and Parak, W.J., Controlled antibody/(bio-) conjugation of inorganic nanoparticles for targeted delivery, Adv. Drug Deliv. Rev., 2013, vol. 65, no. 5, pp. 677–688.
Eby, D.M., Luckarift, H.R., and Johnson, G.R., Hybrid antimicrobial enzyme and silver nanoparticles coatings for medical instruments, Appl. Mater. Interfaces, 2009, vol. 1, no. 7, pp. 1553–1560.
Mei, L., Lu, Z., Zhang, W., Wu, Z., Zhang, X., Wang, Y., Luo, Y., Li, C., and Jia, Y., Bioconjugated nanoparticles for attachment and penetration into pathogenic bacteria, Biomaterials, 2013, vol. 34, pp. 10328–10337.
Krutyakov, Yu.A., Kudrinskii, A.A., Olenin, A.Yu., and Lisichkin, G.V., Synthesis and properties of silver nanoparticles: advances and prospects, Russ. Chem. Rev., 2008, vol. 77, no. 3, pp. 233–257.
Parsaev, A.A., Abkhalimov, E.V., Yakimov, E.E., and Ershov, B.G., Preparation of silver nanoparticles in aqueous solutions in the presence of carbonate ions as stabilizers, Colloid. J., 2011, vol. 73, no. 1, pp. 1–5.
Sergeev, G.B., Nanokhimiya (Nanochemistry), Moscow: Mosk. Gos. Univ., 2003.
Golubeva, O.Yu., Shamova, O.V., Orlov, D.S., Pazina, T.V., Boldina, A.S., Drozdova, I.A., and Kokryakov, V.N., Synthesis and study of antimicrobial activity of bioconjugates of silver nanoparticles and endogenous antibiotics, Glass Phys. Chem., 2011, vol. 37, no. 1, pp. 78–84.
Orlov, D.S., Shamova, O.V., Golubeva, O.Yu., Pazina, T.Yu., Yamshchikova, E.V., Kolodkin, N.I., Kokryakov, V.N., and Korneva, E.A., Action of complexes of natural antimicrobial peptides and silver nanoparticles on microorganisms, Tsitokin. Vospal., 2010, vol. 9, no. 2, pp. 32–36.
Yakovlev, A.V. and Golubeva, O.Yu., Optimization of the synthesis and study of stable aqueous dispersions of silver nanoparticles used in medicine, Glass Phys. Chem., 2013, vol. 39, no. 6, pp. 643–648.
Golubeva, O.Yu., Synthesis optimization of lysozyme manolayer-coated silver nanoparticles in aqueous solution, J. Nanomater., 2014. doi 10.1155/2014/460605
Svergun, D.I. and Feigin, L.A., Rentgenovskoe i neitronnoe malouglovoe rasseyanie (X-ray and Neutron Small-Angle Scattering), Moscow: Nauka, 1986.
Lehrer, R.I., Rosenman, M., Harwig, S.S.L., Jackson, R., and Eisenhauer, P., Ultrasensitive assays for endogenous antimicrobial poly-peptides, J. Immunol. Methods, 1991, vol. 137, pp. 167–173.
National Committee for Clinical Laboratory Standards, Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically, 5th ed., Approved Standard M7-A5, Wayne, PA: Natl. Committee for Clinical Laboratory Standards, 2002.
Golubkov, V.V., Features of structure and its relaxations in alkali borate glasses, Glass Phys. Chem., 1996, vol. 22, no. 3, pp. 186–195.
Ushakov, E.V., Golubkov, V.V., Litvin, A.P., Parfenov, P.S., and Baranov, A.V., Self-organization of lead sulfide quantum dots of different sizes, Nauch.-Tekh. Vestn. Inform. Tekhnol., Mekh. Opt., 2013, vol. 88, no. 6, pp. 127–132.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © O.Yu. Golubeva, V.V. Golubkov, V.A. Yukhnev, O.V. Shamova, 2017, published in Fizika i Khimiya Stekla.
Rights and permissions
About this article
Cite this article
Golubeva, O.Y., Golubkov, V.V., Yukhnev, V.A. et al. Synthesis of silver nanoparticles modified with lysozyme and sodium dioctyl sulfosuccinate and comparison of their biological activities. Glass Phys Chem 43, 63–69 (2017). https://doi.org/10.1134/S1087659617010060
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1087659617010060