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  • Proceedings of the Topical Meeting of the European Ceramic Society “Structural Chemistry of Partially Ordered Systems, Nanoparticles, and Nanocomposites” (St. Petersburg, Russia, June 27–29, 2006)
  • Published:

Nanostructure ordering of proteoglycans of some biological objects

Abstract

This paper reports on the results of systematic X-ray diffraction investigations into the structure of proteoglycans of the mucus and the extracellular matrix of biological tissues. The small-angle X-ray diffraction patterns of different native and modified tissues of man and animals are measured using synchrotron radiation. A comparative study of the structure of mucins from the mammal and invertebrate mucus is performed. It is demonstrated that the X-ray diffraction patterns of the mammal and invertebrate mucus are very similar to each other and exhibit a number of sharp diffraction rings with a spacing of 4.5 nm and its higher orders due to the highly ordered structure of giant proteoglycan molecules. It is revealed that the proteoglycan structure undergoes a transformation upon interaction with calcium cations. The type of X-ray diffraction pattern (“with a 4.5-nm ring” and “without a ring”) for intact tissues can be reversibly changed by treating the tissues in solutions of salts or chelating agents. The inference is made that the X-ray diffraction patterns of tissues can be used as markers for the structural transformation of the extracellular matrix under different exogenous actions.

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References

  1. 1.

    Aksirov, A.M., Gerasimov, V.S., Kondratyev, V.I., Korneev, V.N., Kulipanov, G.N., Lanina, N.F., Letyagin, V.P., Mezentsev, N.A., Sergienko, P.M., Tolochko, B.P., Trounova, V.A., and Vazina, A.A., Biological and Medical Application of SR from the Storage Rings of VEPP-3 and “Siberia-2:” The Origin of Specific Changes of Small-Angle X-Ray Diffraction Pattern of Hair and Their Correlation with the Elemental Content, Nucl. Instrum. Methods Phys. Res., Sect. A, 2001, vol. 470, pp. 380–387.

  2. 2.

    Vazina, A.A., Gerasimov, V.S., Gorbunova, N.P., Sergienko, P.M., Shelestov, V.M., Nesterikhin, Yu.I., Baryshev, V.B., Zolotarev, K.V., Kulipanov, G.N., and Trounova, V.A., Noninvasive Methods for Express Analysis of Biological Objects Based on Elemental Analysis Using Synchrotron Radiation on Hair Samples from Animals and Patients, Nucl. Instrum. Methods Phys. Res., Sect. A 1998, vol. 405, pp. 454–458.

  3. 3.

    Vazina, A.A., Bras, W., Dolbnya, I.P., Korneev, V.N., Lanina, N.F., Matyushin, A.M., Sergienko, P.M., and Zabelin, A.V., Peculiarities of Human Hair Structural Dynamics, Nucl. Instrum. Methods Phys. Res., Sect. A, 2005, vol. 543, pp. 153–157.

  4. 4.

    Vazina, A.A., Budantsev, A.Yu., Bras, W., Deshcherevskaya, N.P., Dolbnya, I.P., Gadzhiev, A.M., Korneev, V.N., Lanina, N.F., Letyagin, V.P., Maevsky, E.I., Matyushin, A.M., Podolsky, I.Ya., Samsonova, M.V., Sergienko, P.M., Simonova, N.B., Stankevich, V.G., Trunova, V.A., Vavilov, A.M., Chernyaev, A.L., Sharafutdinov, M.R., and Sheromov, M.A., X-Ray Diffraction and Spectral Studies of Biological Native and Modified Tissues, Nucl. Instrum. Methods Phys. Res., Sect. A, 2005, vol. 543, pp. 297–301.

  5. 5.

    Denisova, E.A., Lazarev, P.I., Vazina, A.A., and Zhelesnaya, L.A., Intestinal Mucus and Juice Glycoproteins Have a Liquid Crystalline Structure, Stud. Biophys., 1985, vol. 108, no. 2, pp. 117–124.

  6. 6.

    Skopinov, A.A., Yakovleva, S.V., Denisova, E.A., Vazina, A.A., and Zheleznaya, L.A., The Influence of Low-Intensity Laser Radiation on Liquid-Crystal Structures Formed in a Glycoprotein Solution, Mol. Biol. (Moscow), 1989, vol. 23, pp. 416–420.

  7. 7.

    Mintz, R.I., Skopinov, A.S., Yakovleva, S.V., Denisova, E.A., Vazina, A.A., Zheleznaya, L.A., and Lazarev, P.I., Formation of Oriented Films by Mucus Glycoproteins on the Faces of NaCl Crystal, Stud. Biophys., 1989, vol. 133, pp. 221–225.

  8. 8.

    Ariskin, N.I., Gerasimov, V.S., Korneev, V.N., Sergienko, P.M., Shishkov, V.I., Sheromov, M.A., Stankevich, V.G., and Vazina, A.A., System of Primary Collimators of SR Beam at the Small-Angle Station for KSRS, Nucl. Instrum. Methods Phys. Res., Sect. A, 2001, vol. 470, pp. 118–121.

  9. 9.

    Korneev, V.N., Sergienko, P.M., Matyushin, A.M., Shlektarev, V.A., Ariskin, N.I., Shishkov, V.I., Gorin, V.P., Sheromov, M.A., Aul’chenko, V.M., Zabelin, A.V., Stankevich, V.G., Yudin, L.I., and Vazina, A.A., Current Status of the Small-Angle Station at Kurchatov Center of Synchrotron Radiation, Nucl. Instrum. Methods Phys. Res., Sect. A, 2005, vol. 543, pp. 368–374.

  10. 10.

    Vazina, A.A., Denisova, E.A., Zheleznaya, L.A., and Lazarev, P.I., X-Ray Diffraction Investigation of Glycoproteins Separated from Canine Duodenal Juice, Dokl. Akad. Nauk SSSR, 1985, vol. 281, no. 4, pp. 975–978.

  11. 11.

    Vasil’eva, A.V., Vazina, A.A., Matyushin, A.M., Lanina, N.F., and Zabelin, A.V., Structural Features of Mucin-Like Mucus of South American Humboldt Squid as a Representative of Invertebrates (Preliminary X-Ray Diffraction Study), Tezisy dokladov V Natsional’noi konferentsii po primeneniyu rentgenovskogo sinkhrotronnogo izlucheniya, neitronov i elektronov RSNE-NANO-2005 (Extended Abstracts of Papers of the V National Conference on Application of X-Ray Synchrotron Radiation, Neutrons, and Electrons RSNE-NANO-2005), Moscow, 2005, p. 86 [in Russian].

  12. 12.

    Trounova, V.A., Vazina, A.A., Lanina, N.F., Sergienko, P.M., and Kondratyev, V.I., Correlation between Element Concentrations and X-Ray Diffraction Patterns in Hair, X-Ray Spectrom., 2002, vol. 31, pp. 314–318.

  13. 13.

    Vazina, A.A., Gadzhiev, A.M., Sergienko, P.M., Gerasimov, V.S., and Korneev, V.N., Time-Resolved Small-Angle X-Ray Diffraction from Contracting Muscle, Rev. Sci. Instrum., 1989, vol. 60, no. 7, pp. 2350–2353.

  14. 14.

    Vazina, A.A., Gadzhiev, A.M., Gerasimov, V.S., Gorbunova, N.P., Sergienko, P.M., Korneev, V.N., Aulchenko, V.M., and Baru, S.E., The Use of the Time-Resolved X-Ray Diffraction and Sample Techniques for Studying the Muscle Structure during Relaxation, Nucl. Instrum. Methods Phys. Res., Sect. A 1995, vol. 359, pp. 210–215.

  15. 15.

    Zheleznaya, L.A., Kostyuchenko, L.N., Denisova, E.A., and Gerasimov, V.S., X-Ray Diffraction Study of Adaptive Changes in the Structure of Dense Phase Juice from the Duodenum, Biofizika, 1994, vol. 39, pp. 911–914 [Biophysics (Engl. transl.), 1994, vol. 39, pp. 891–894].

  16. 16.

    Zheleznaya, L.A., Denisova, E.A., Kostyuchenko, L.N., and Gerasimov, V.S., Roentgenographic Study of the Duodenal Juice Components in the Dog, Biofizika, 1997, vol. 42, pp. 234–238 [Biophysics (Engl. transl.), 1997, vol. 42, pp. 221–225].

  17. 17.

    Gal’perin, Yu.M., Kostyuchenko, L.N., Zheleznaya, L.A., and Denisova, E.A., Role of Glycoproteins in the Formation of Floccular Gel Structures of Natural Digestion, Fiziol. Zh. SSSR im. I.M. Sechenova, 1990, vol. 76, pp. 1068–1071.

  18. 18.

    Zheleznaya, L.A., Denisova, E.A., Lazarev, P.I., and Vazina, A.A., X-Ray Diffraction Studies on Fine Structure of Mucus Glycoprotein, J. Nanobiol., 1992, vol. 1, pp. 107–115.

  19. 19.

    Marshall, J.J., Manipulation of the Properties of Enzymes by Covalent Attachment of Carbohydrate, Trends Biochem. Sci., 1978, vol. 3, p. 79.

  20. 20.

    James, V., Kearsley, J., Irving, T., Amemiya, Y., and Cookson, D., Using Hair to Screen for Breast Cancer, Nature (London), 1999, vol. 398, pp. 33–34.

  21. 21.

    James, V., False-Positive Results in Studies of Changes in Fiber Diffraction of Hair from Patients with Breast Cancer May Not Be False, J. Natl. Cancer Inst., 2003, vol. 5, pp. 170–171.

  22. 22.

    Briki, F., Busson, B., Salicru, B., Esteve, F., and Doucet, J., Breast-Cancer Diagnosis Using Hair, Nature (London), 1999, vol. 400, p. 226.

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Original Russian Text © A.A. Vazina, N.F. Lanina, V.N. Korneev, A.M. Matyushin, A.V. Zabelin, O.V. Nayda, E.I. Maevsky, E.P. Polyakova, 2007, published in Fizika i Khimiya Stekla.

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Vazina, A.A., Lanina, N.F., Korneev, V.N. et al. Nanostructure ordering of proteoglycans of some biological objects. Glass Phys Chem 33, 294–301 (2007). https://doi.org/10.1134/S1087659607030194

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Keywords

  • Synchrotron Radiation
  • Hair Sample
  • Glass Physic
  • Hair Length
  • Duodenal Juice