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Synthesis of a Deuterium-Labeled Globotriaosylceramide Probe for Potential Imaging of Subcellular Localization of Gb3 Using NanoSIMS

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Abstract

Globotriaosylceramide (Gb3) is a cell membrane glycosphingolipid expressed by renal cells and gastrointestinal tract and overexpressed by many carcinomas. Subcellular distribution of this biomarker is highly significant for the development of drugs and therapy of diseases related to its interactions with cell surface. This paper describes a simple method for the synthesis of a deuterium-labeled fatty acyl side chain in its ceramide residue as a probe for those who are interested in NanoSIMS 50-based imaging technique in structural biology.

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ACKNOWLEDGMENTS

The authors are grateful to the Deanship of Scientific Research at the Taif University (Taif, Saudi Arabia) for funding and support according to project no. TURSP2020/27 and to the Curie Institute (Paris, France) for supporting and facilitating performing this study.

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Authors and Affiliations

  1. Chemistry Department, Faculty of Science, Port Said University, 42522, Port Said, Egypt

    M. R. E. Aly

  2. Food Science & Nutrition Department, College of Science, Taif University, 21944, Taif, Saudi Arabia

    I. H. El Azab

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  1. M. R. E. Aly
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  2. I. H. El Azab
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Correspondence to M. R. E. Aly.

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Aly, M.R.E., Azab, I.H.E. Synthesis of a Deuterium-Labeled Globotriaosylceramide Probe for Potential Imaging of Subcellular Localization of Gb3 Using NanoSIMS. Russ J Org Chem 57, 1719–1724 (2021). https://doi.org/10.1134/S1070428021100213

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