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Improvement of ways to obtain ethidium bromide and synthesis of ethidium ethyl sulfate, a new fluorescent dye for detection of nucleic acids

Russian Journal of Applied Chemistry volume 84, Article number: 1541 (2011) Cite this article

Abstract

Two methods for synthesis of ethidium bromide, a fluorescent dye widely used in molecular biology, were improved. An analog of ethidium bromide, ethidium ethyl sulfate, was synthesized.

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References

  1. Sambrook, J., Fritsch, E.F., and Maniatis, T., Molecular Cloning, Cold Spring Harbor Laboratory Pr, 1989.

  2. US Patent 4683202.

  3. Using Molecular Marker Technology in Studies on Plant Genetic Diversity, DNA-based Technologies, PCR-based Technologies, Sequence-tagged Sites (Microsatellites, SCARs, CAPS, ISSRs), IPGRI and Cornell University, 2003.

  4. Karsten, U. and Wollenberger, A., Anal. Biochem., 1977, vol. 77, pp. 464–470.

    Article  CAS  Google Scholar 

  5. Giache, V., Pirami, L., and Becciolini, A., J. Biolumin. Chemilumin., 1994, vol. 9, no. 3, pp. 329–332.

    Article  Google Scholar 

  6. GB Patent 735438.

  7. US Patent 2662082.

  8. RF Patent 2396260.

  9. Firth, W.J., Watkins, C.L., Graves, D.E., and Yielding, L.W., J. Heterocycl. Chem., 1983, vol. 20, no. 3, pp. 759–765.

    Article  CAS  Google Scholar 

  10. RF Patent 2415842.

  11. DRP Patent 602 698.

  12. Spravochnik khimika (Chemist’s Handbook), vol. 2, Nikol’skii, B.P., ED., Leningrad: Khimiya, 1964, p. 656.

    Google Scholar 

  13. Semikolenov, V.A., Simakova, I.L., and Sadovnichii, G.V., Khim. Prom-st’, 1966, no. 3, pp. 184–191.

  14. Organic Syntheses, Collective Volume 5, Baumgarten, H.E., Ed., New York: J. Wiley and Sons, 1973.

    Google Scholar 

  15. Shchori, E., Jagur-Grodzinski, J., and Shporer, M., J. Am. Chem. Soc., 1973, vol. 95, pp. 3842–3846.

    Article  CAS  Google Scholar 

  16. Morgan, G.T. and Walls, L.P., J. Soc. Chem. Ind., 1930, p. 49, p. 15T.

  17. Cymerman, J. and Short, W.F., J. Chem. Soc., 1949, pp. 703–707.

  18. Konakahara, T., Wurdeman, R.L., and Gold, B., Biochemistry, 1988, vol. 27, no. 23, pp. 8606–8613.

    Article  CAS  Google Scholar 

  19. Barber, H.J., Bretherick, L., Elridge, E.M., et al., J. Soc. Chem. Ind., 1950, vol. 69, no. 3, pp. 82–96.

    Article  CAS  Google Scholar 

  20. Yur’ev, Yu.K., Prakticheskie raboty po organicheskoi khimii (Practical Works in Organic Chemistry), issues 1 and 2, Moscow: Mosk. Gos. Univ., 1964.

    Google Scholar 

  21. Watkins, T.I., J. Chem. Soc., 1952, pp. 3059–3064.

  22. Luedtke, N.W., Liu, Q., and Tor, Y., Chemistry A European J., 2005, vol. 11, no. 2, pp. 495–508.

    Article  CAS  Google Scholar 

  23. Ross, S.A., Pitie, M., and Meunier, B., J. Chem. Soc., Perkin Trans. 1, 2000, no. 4, pp. 571–574.

  24. Rangarajan, S. and Friedman, S.H., Bioorg. Med. Chem. Lett., 2007, vol. 17, no. 8, pp. 2267–2273.

    Article  CAS  Google Scholar 

  25. Aldrich, Katalog-spravochnik khimicheskikh reaktivov, Rossiya, 2007–2008 (Aldrich, Catalogue-Reference Book of Chemical Reagents, Russia, 2007–2008).

  26. The Sadtler Standard Spectra: Infrared Grating Spectra, Philadelphia, 1966–1989, Spectrum N 22, 948.

  27. The Sadtler Standard Spectra: Ultraviolet Spectra. Philadelphia, 1966–1968, Spectrum N 28, 880.

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Authors and Affiliations

  1. Vorozhtsov Institute of Organic Chemistry, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia

    S. A. Osadchii, V. G. Shubin, L. P. Kozlova & V. S. Varlamenko

  2. Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia

    M. L. Filipenko & U. A. Boyarskikh

Authors
  1. S. A. Osadchii
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  2. V. G. Shubin
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  3. L. P. Kozlova
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  4. V. S. Varlamenko
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  5. M. L. Filipenko
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  6. U. A. Boyarskikh
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Additional information

Original Russian Text © S.A. Osadchii, V.G. Shubin, L.P. Kozlova, V.S. Varlamenko, M.L. Filipenko, U.A. Boyarskikh, 2011, published in Zhurnal Prikladnoi Khimii, 2011, Vol. 84, No. 9, pp. 1491–1498.

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Osadchii, S.A., Shubin, V.G., Kozlova, L.P. et al. Improvement of ways to obtain ethidium bromide and synthesis of ethidium ethyl sulfate, a new fluorescent dye for detection of nucleic acids. Russ J Appl Chem 84, 1541 (2011). https://doi.org/10.1134/S107042721109014X

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  • DOI: https://doi.org/10.1134/S107042721109014X

Keywords

  • Ethidium Bromide
  • Nitrobenzene
  • Elemental Analysis Data
  • Main Substance
  • Hydrobromic Acid