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Stabilization of Double-Stranded Poly(A)·Poly(U) with ZnTMPyP4 Metalloporphyrin in Aqueous Solution

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Abstract

UV-Vis absorption spectra of aqueous solutions containing both metalloporphyrin Zn(X)TMPyP4 [H2TMPyP4—5,10,15,20-tetrakis(1-methylpyridin-4-yl)-21H,23H-porphyrin] and synthetic polyadenylic-polyuridylic acid Poly(A)·Poly(U) over the temperature range from 20 to 70°C (pH = 7.0, I = 0.15 M.) have been analyzed. Deconvolution of the spectrometric data matrix, without postulating a physicochemical equilibrium model, has allowed estimation of the contribution of the Poly(A)·Poly(U)·(ZnTMPyP4)n complex to the total change in the spectra. Chemometric analysis has shown an increase in the melting temperature of this ternary complex by 9.4°С compared to pure polyribonucleotide, which indicates the stabilization of the bonds between nucleic bases in the Poly(A)·Poly(U) polynucleotide under the influence of bound porphyrin.

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  1. St. Petersburg State University, 190034, St. Petersburg, Russia

    A. G. Kudrev

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Kudrev, A.G. Stabilization of Double-Stranded Poly(A)·Poly(U) with ZnTMPyP4 Metalloporphyrin in Aqueous Solution. Russ J Gen Chem 90, 2281–2288 (2020). https://doi.org/10.1134/S1070363220120105

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