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Spectrometric study of the oligodeoxyribonucleotide protonation in aqueous solution

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Abstract

UV absorption spectra and circular dichroism spectra of aqueous solutions of cytosin- and thyminecontaining single-stranded Oligodeoxyribonucleotide 5′-CCTTTCCTTTTCCTTTCC-3′(ckit4) were measured at various pH in the range 3.3–8.9. The chemometric analysis of the multiinstrumental data matrix was carrie out. The diagrams of relative contents of complex forms of the DNA molecule absorbing in the studied wavelength range (220–320 nm) were constructed by the ALS-MCR soft simulation procedure without initial postulation of their chemical compositions. The model of equilibrium complex formation describing observed changes in the spectra depending on the solution acidity was developed on the basis of the matrix method. Intrinsic protonation constants of the oligonucleotide ckit4 were calculated. The formation of intramolecular complexes between cytosine C.C+ bases in the studied DNA molecule is of a cooperative nature, and thei subsequent protonation is an anticooperative process.

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Authors and Affiliations

  1. University of Barcelona, Barcelona, Spain

    R. Gargallo

  2. Institute of Structural Biology, Barcelona, Spain

    R. Eritja

  3. St. Petersburg State University, Universitetskii pr. 26, St. Petersburg, 198504, Russia

    A. G. Kudrev

Authors
  1. R. Gargallo
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  2. R. Eritja
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  3. A. G. Kudrev
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Correspondence to A. G. Kudrev.

Additional information

Original Russian Text © R. Gargallo, R. Eritja, A.G. Kudrev, 2010, published in Zhurnal Obshchei Khimii, 2010, Vol. 80, No. 3, pp. 462–470.

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Gargallo, R., Eritja, R. & Kudrev, A.G. Spectrometric study of the oligodeoxyribonucleotide protonation in aqueous solution. Russ J Gen Chem 80, 485–492 (2010). https://doi.org/10.1134/S1070363210030205

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  • DOI: https://doi.org/10.1134/S1070363210030205

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