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Biotechnology. Gene expression and microchips: Problems of the quantitative analysis

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Abstract

The technology of gene microchips that performed a real revolution in the investigation of the genetic system [1] affected the development of many medical and biological branches of science, including oncology [2, 3], toxicology [4], pharmacology [5], biology of development [6], facilitated the solution of classical problems of molecular biology concerning the study of gene mutations and transcription mechanisms [7, 8]. In contrast to the traditional approach of the molecular genetics, like the methods based on the polymerase chain reaction (PCR), Nothern-blotting and the subsequent analysis of the gene expression [9–11], addressed to the study of expression of a single or few genes, the microchips made it possible to carry out simultaneous monitoring of the expression of the whole cell genome under strictly controlled conditions. This monitoring provides a possibility to reveal the strategies used by the cell in response to the change in the external conditions, to establish the groups of genes functionally connected, to reconstruct the mechanisms of transcription regulation and to determine the metabolic ways connected thereto, to analyze the genes whose function in the cell remained unknown.

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  1. Physical Department, Lomonosov Moscow State University, Leninskie Gory, Moscow, 119992, Russia

    A. N. Sveshnikova & P. S. Ivanov

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  1. A. N. Sveshnikova
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Original Russian Text © A.N. Sveshnikova, P.S. Ivanov, 2007, published in Russkii Khimicheskii Zhurnal, 2007, Vol. 51, No. 1, pp. 127–135.

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Sveshnikova, A.N., Ivanov, P.S. Biotechnology. Gene expression and microchips: Problems of the quantitative analysis. Russ J Gen Chem 77, 2071–2081 (2007). https://doi.org/10.1134/S1070363207110369

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