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Extraction of Flavonoids from Koenigia weyrichii Using a Deep Eutectic Mixture of Choline Chloride + Glycerine

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Abstract—

Koenigia weyrichii (F. Schmidt) T.M. Schust. et Reveal, which grows on the Kola Peninsula, contains a significant amount of flavonoids in the aerial parts of the plant, rapidly builds up biomass, and is resistant to various climatic conditions. This makes K. weyrichii a promising source of biologically active substances (BAS) in the Arctic zone. A relatively new method for extracting biologically active substances from plants is the use of deep eutectic solvents (DES), for example, a mixture of choline chloride with glycerol, which has shown high efficiency in the extraction of flavonoids from other plants. Previously, DES was not used for the extraction of biologically active substances from K. weyrichii. The aim of this work is to evaluate the efficiency of ultrasonic extraction using traditional solvents (water, ethanol) and DES choline chloride + glycerol and to optimize the extraction conditions. Comparison of the total content of polyphenols, flavonoids, antioxidative, and antiradical activity in various extracts is carried out. It was found that the most favorable conditions for extraction are: water content in DES of 17.5 wt %, a temperature of 65°C, and a time of three hours. At the same time, under identical extraction conditions (temperature and time), more target substances can be extracted with 60% ethanol than with DES. However, DES can be seen as an alternative to using ethanol. The data obtained can be useful for the further development of innovative technologies of the extraction of biologically active substances from plant material. Also, K. weyrichii may be considered as a source of biologically active substances in cosmetics and the food industry.

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Funding

This work was financially supported by a grant from the President of the Russian Federation MK-566.2020.11.

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Authors and Affiliations

  1. Tananaev Institute of Chemistry and Technology of Rare Elements and Mineral Resources, Federal Research Center Kola Science Center, Russian Academy of Sciences, 184209, Apatity, Russia

    N. S. Tsvetov

  2. Laboratory of Medical and Biological Technologies, Federal Research Center Kola Scientific Center, Russian Academy of Sciences, 184209, Apatity, Russia

    A. V. Korovkina

  3. Geological Institute, Federal Research Center Kola Science Center, Russian Academy of Sciences, 184209, Apatity, Russia

    O. I. Paukshta

Authors
  1. N. S. Tsvetov
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  2. A. V. Korovkina
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  3. O. I. Paukshta
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Correspondence to N. S. Tsvetov.

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The authors declare that they have no conflicts of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Tsvetov, N.S., Korovkina, A.V. & Paukshta, O.I. Extraction of Flavonoids from Koenigia weyrichii Using a Deep Eutectic Mixture of Choline Chloride + Glycerine. Russ J Bioorg Chem 48, 1565–1570 (2022). https://doi.org/10.1134/S1068162022070275

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