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Chemical Composition and Biological Activity of Rosmatin, a Dry Extract from Dracocephalum moldavica L.

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Abstract

Moldavian dragonhead (Dracocephalum moldavica L.) is an annual herb. A new variety (Nezhnost) of this herb has been bred at the All-Russian Research Institute of Medicinal and Aromatic Plants (ARRIMAP), and a technology for obtaining a dry purified extract named “Rosmatin” has been developed. The NMR (1H and 13C) and HPLC-UV-MS analysis of the extract using standard samples and published data has determined the qualitative and quantitative composition of Rosmatin. The following phenolic compounds have been identified: rosmarinic, caffeic, and ferulic acids; rosmarinic acid 3-O-glucoside; luteolin-7-O-β-D-glucuronide; acacetin-7-O-β-D-glucopyranoside (tilianin); apigenin-7-O-β-D-glucoside (cosmosiin); apigenin-7-O-β-D-glucuronide; apigenin-7-O-β-D-(6''-O-malonyl) glucoside; chrysoeryol-7-O-β-D-glucuronide; acacetin-7-O-β-D-glucuronide; acacetin-7-O-β-D-(6''-malonyl) glucoside; and schizotenuin F. The content of rosmarinic acid in the Rosmatin extract is 56.6%, and the total content of flavonoids is ~27%. The anti-inflammatory and gastroprotective activity of Rosmatin at the dosages of 10 and 100 mg/kg has been studied. A dose-dependent anti-inflammatory effect with the suppressed development of the exudative phase of inflammation as well as a dose-dependent gastroprotective effect significantly exceeding that of the reference drug (omeprazole) has been revealed. As found earlier, Rosmatin also has a tonic effect on the cardiovascular and nervous systems.

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Authors and Affiliations

  1. All-Russian Research Institute of Medicinal and Aromatic Plants, 117216, Moscow, Russia

    O. P. Sheychenko, V. I. Sheychenko, E. V. Zvezdina, E. N. Kurmanova, E. V. Ferubko & E. V. Uyutova

  2. Peoples’ Friendship University of Russia, 117198, Moscow, Russia

    S. V. Goryainov, O. G. Potanina & K. Fadi

Authors
  1. O. P. Sheychenko
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  2. V. I. Sheychenko
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  3. S. V. Goryainov
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  4. E. V. Zvezdina
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  5. E. N. Kurmanova
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  6. E. V. Ferubko
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  7. E. V. Uyutova
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  8. O. G. Potanina
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Correspondence to O. P. Sheychenko.

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Statement on the welfare of animals. Experiments were performed in accordance with the GLP principles, Order no. 199 of the Ministry of Health of the Russian Federation On Approval of Laboratory Practice Rules (2016), and Guidelines for Preclinical Drug Studies (Moscow, 2012). The study was approved by the ARRIMAP Bioethics Commission.

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Translated by N. Statsyuk

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Sheychenko, O.P., Sheychenko, V.I., Goryainov, S.V. et al. Chemical Composition and Biological Activity of Rosmatin, a Dry Extract from Dracocephalum moldavica L.. Russ J Bioorg Chem 48, 1550–1559 (2022). https://doi.org/10.1134/S1068162022070251

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  • DOI: https://doi.org/10.1134/S1068162022070251

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