Skip to main content
SpringerLink
Log in

Development of a Method of Quantitative Determination of Total Flavonoids in the Leaves of Myrtus communis

  • Published:
Russian Journal of Bioorganic Chemistry Aims and scope Submit manuscript

Abstract—

Myrtle (Myrtus communis L.) is an evergreen plant, a small tree or shrub endemic to the Mediterranean region. In the area of growth since ancient times, various parts (berries, fruits and leaves) of myrtle have been used in folk medicine. The therapeutic properties of varies parts of the myrtle can be caused by different biologically active compounds, such as terpenoids (α-pinene, 1,8-cineole, geranyl acetate, linalool, limonene, trans-myrtanol acetate), polyphenolic compounds named flavonoids (quercetin, myricetin, myrtocommulones, etc.) Currently, myrtle is successfully cultivated in the coastal zone of the southern coast of Crimea. We studied samples of leaves of myrtle, harvested in the Nikitsky Botanical Garden, National Science Center, Russian Academy of Sciences. The optimal conditions for the extraction of flavonoids from the leaves were determined. It was established that the appropriate extragent for this raw material is 80% ethanol. The extraction time is 90 min, the ratio of “raw material–extragent” is 1 : 50. The method has been developed for the quantitative determination of the total flavonoids (calculated on rutin) in the leaves of myrtle by the method of differential spectrophotometry, at an analytical wavelength of 414 nm. Using the developed methodology, a number of samples of myrtle leaves were analyzed, and it was established that the content of the total flavonoids in the leaves of myrtle varies from 2.48 ± 0.08% to 3.64 ± 0.12% (calculated on rutin).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.

Similar content being viewed by others

REFERENCES

  1. Aleksic, V. and Knezevic, P., Antimicrobial and antioxidative activity of extracts and essential oils of Myrtus communis L., Microbiol. Res., 2014, vol. 169, no. 4, pp. 240–254.

    Article  CAS  Google Scholar 

  2. Hennia, A., Miguel, M.G., and Nemmiche, S., Antioxidant activity of Myrtus communis L. and Myrtus nivellei Batt. & Trab. extracts: A brief review, Medicines, 2018, vol. 5, no. 3, p. 89.

    Article  CAS  Google Scholar 

  3. Migliore, J., Baumel, A., Juin, M., and Medail, F., From mediterranean shores to Central Saharan mountains: Key phylogeographical insights from the genus myrtus, J. Biogeogr., 2012, no. 39, pp. 942–956.

  4. Yoshimura, M., Amakura, Y., Tokuhara, M., and Yoshida, T., Polyphenolic compounds isolated from the leaves of Myrtus communis, J. Nat. Med., 2008, vol. 62, no. 3, pp. 366–368.

    Article  CAS  Google Scholar 

  5. Alipour, G., Dashti, S., and Hosseinzadeh, H., Review of pharmacological effects of Myrtus communis L. and its active constituents, Photother. Res., 2014, vol. 28, no. 8, pp. 1125–1136.

    Article  CAS  Google Scholar 

  6. Bakova, E.Yu., Palii, A.E., Bakova, N.N., and Fedotova, I.A., The features of the component composition of Myrtus communis L. essential oil, grown under the conditions of the southern coast of the Crimea, Vopr. Biol. Med. Farm. Khim., 2018, vol. 21, no. 11, pp. 30–35.

    CAS  Google Scholar 

  7. Chalchat, J.-C., Garry, R.-P., and Michet, A., Essential oils of myrtle (Myrtus communis L.) of the mediterranean littoral, J. Essent. Oil Res., 1998, vol. 10, pp. 613–617.

    Article  CAS  Google Scholar 

  8. Flamin, G., Cioni, P.L., Morelli, I., Maccioni, S., and Baldini, R., Phytochemical typologies in some populations of Myrtus communis L. on Caprione Promontory (East Liguria, Italy), Food Chem., 2004, vol. 85, no. 4, pp. 599–604.

    Article  Google Scholar 

  9. Bakova, N.N., Bakova, E.Yu., Palii, A.E., and Konovalov, D.A., Biologically active compounds of Myrtus communis L., in Biotekhnologiya kak instrument sokhraneniya bioraznoobraziya rastitel’nogo mira (fiziologo-biokhimicheskie, embriologicheskie, geneticheskie i pravovye aspekty) (Biotechnology as a Tool for the Conservation of Plant Biodiversity: Physiological, Biochemical, Embryological, Genetic and Legal Aspects, Proceedings of the 8th International Conference), 2018, pp. 121–122.

  10. Feißt, C., Franke, L., Appendino, G., and Werz, O., Identification of molecular targets of the oligomeric onprenylated acylphloroglucinols from Myrtus communis and their implication as anti-inflammatory compounds, J. Pharmacol. Exp. Therapeut., 2005, vol. 315, no. 1, pp. 389–396.

    Article  Google Scholar 

  11. Gerbeth, K., Hüsch, J., Meins, J., Rossi, A., Sautebin, L., Wiechmann, K., Werz, O., Skarke, C., Barrett, J.S., Schubert-Zsilavecz, M., and Abdel-Tawab, M., Myrtucommulone from Myrtus communis: Metabolism, permeability, and systemic exposure in rats, Planta Med., 2012, no. 78, pp. 1932–1938.

  12. Gerbeth, K., Meins, J., Werz, O., Schubert-Zsilavecz, M., and Abdel-Tawab, M., Determination of myrtucommulone from Myrtus communis in human and rat plasma by liquid hromatography/tandem mass spectrometry, Planta Med., 2011, no. 77, pp. 450–454.

  13. Hans, M., Charpentier, M., Huch, V., Jauch, J., Bruhn, T., Bringmann, G., and Quandt, D., Stereoisomeric composition of natural myrtucommulone A, J. Nat. Products, 2015, no. 78, pp. 2381–2389.

  14. Koeberle, A., Pollastro, F., Northoff, H., and Werz, O., Myrtucommulone, a natural acylphloroglucinol, inhibits microsomal prostaglandin E2 synthase-1, Brit. J. Pharmacol., 2009, no. 156, pp. 952–961.

  15. Nicoletti, R., Ferranti, P., Caira, S., Misso, G., Castellano, M., Di Lorenzo, G., and Caraglia, M., Myrtucommulone production by a strain of neofusicoccum australe endophytic in myrtle (Myrtus communis), World J. Microbiol. Biotechnol., 2014, no. 30, pp. 1047–1052.

  16. Pharmacopee Francaise, 11 ed., pp. 2012–2015.

  17. State Register of Medicines. https://grls.rosminzdrav. ru/Default.aspx. Accessed September 2, 2019.

  18. Gosudarstvennaya farmakopeya Rossiiskoi Federatsii (State Pharmacopoeia of the Russian Federation), 14th ed., Moscow: Min. Zdravookhran. RF, 2018. http://femb.ru/femb/pharmacopea.php.

  19. Kurkin, V.A., Osnovy fitoterapii: uchebnoe posobie (Fundamentals of Herbal Medicine, The School-Book), Samara, 2009.

  20. Kurkin, V.A., Farmakognoziya: uchebnik dlya studentov farmatsevticheskikh vuzov (Pharmacognosy, School-Book for Higher School), Samara, 2019.

    Google Scholar 

  21. Kurkin, V.A., Kurkina, A.V., and Avdeeva, E.V., The flavonoids as biologically active compounds of medicinal plants, Fundam. Issled., 2013, no. 11–9, pp. 1897–1901.

  22. Kurkina, A.V., Approaches to the standardization of raw materials containing flavonoids, Vopr. Biol. Med. Farm. Khim., 2013, no. 5, p. 38.

  23. Kurkina, A.V., Flavonoids as a criterion for the quality of medicinal raw materials and preparations of pharmacopoeial plants, in Fenol’nye soedineniya: fundamental’nye i prikladnye aspekty: materialy dokladov VIII Mezhdunarodnogo simpoziuma (Phenolic Compounds: Fundamental and Applied Aspects, Proceedings of the 8th International Symposium), Moscow, 2012, pp. 571–577.

  24. Kurkina, A.V., Flavonoidy farmakopeinykh rastenii: monografiya (Flavonoids of Pharmacopoeial Plants), Samara, 2012.

  25. Kurkina, A.V., Experimental and theoretical substantiation of approaches to the standardization of raw materials and preparations of pharmacopoeial plants containing flavonoids, Extended Abstract of Doctoral (Pharm. Sci.) Dissertation, Samara, 2012.

  26. Kurkin, V.A., Avdeeva, E.V., Kurkina, A.V., et al., The flavonoids as the criteria of the identity and quality of the medicinal plants and phytopharmaceuticals, in Proceedings of the Drug Discovery and Therapy World Congress, Boston, USA, 2013, p. 170.

  27. Kurkin, V.A., Zaitseva, E.N., Pravdivtseva, O.E., Kurkina, A.V., and Stenyaeva, V.V., Fruits of Crataegus submollis: Study of pharmacological activity of the preparations, Nauka Innov. Med., 2019, vol. 4, no. 3, pp. 69–72.

    Article  Google Scholar 

  28. Kupriyanova, E.A. and Kurkin, V.A., The development of approaches to standardization of the Populus nigra leaves, Aspir. Vestn. Povolzh., 2018, nos. 5–6, pp. 17–21.

Download references

Author information

Authors and Affiliations

  1. Samara State Medical University, 443099, Samara, Russia

    V. A. Kurkin, A. I. Khusainova & A. V. Kurkina

  2. Order of the Red Banner of Labor Nikitsky Botanical Gardens–National Scientific Center, Russian Academy of Sciences, 298648, Nikita, Yalta, Russia

    N. N. Bakova & E. Yu. Bakova

Authors
  1. V. A. Kurkin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. I. Khusainova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. V. Kurkina
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. N. N. Bakova
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. E. Yu. Bakova
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to V. A. Kurkin.

Ethics declarations

The authors declare that they have no conflicts of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kurkin, V.A., Khusainova, A.I., Kurkina, A.V. et al. Development of a Method of Quantitative Determination of Total Flavonoids in the Leaves of Myrtus communis. Russ J Bioorg Chem 48, 1470–1475 (2022). https://doi.org/10.1134/S1068162022070159

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1068162022070159

Keywords:

Search

Navigation