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An Identification and a Quantitative Assessment of Phenolic Compounds in a Butanol Fraction of Extract from Cosmary Flowers (Tanacetum vulgare)

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Abstract

The costmary (Tanacetum vulgare L., syn.: Chrysanthemum vulgare (L). Bernh) is a perennial medicinal plant with a Euro-Asian type of a habitat. The costmary extracts have a wide spectrum of a biological and pharmacological activity. A drug with high choleretic and anti-inflammatory activity was created on the basis of the butanol fraction of the extract from the T. vulgare flowers in the All-Russian Institute of Medicinal and Aromatic Plants. The goal of this investigation was a determination of the qualitative composition and the quantitative content of phenolic compounds of the butanol fraction which are considered to be probably responsible for the biological activity of the extract. High performance liquid chromatography with diode-array detection in a combination with high-resolution mass spectrometry (UPLC-PDA-HRMS) was used for the analysis. We demonstrated that seven phenolic compounds were present in the butanol fraction of T. vulgare, including the first-isolated myricetin-3-glucoside. The content of the phenolic compounds achieved 90%, and neochlorogenic, 3,5-, and 4,5-dicaffeoylquinic acids were the main compounds (their content was 69%). Thus, the high content of caffeoylquinic acids could be responsible for the pharmacological activity of the drug based on the butanol fraction of the extract from the costmary flowers.

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Authors and Affiliations

  1. All-Russian Research Institute of Medicinal and Aromatic Plants, 117216, Moscow, Russia

    T. A. Krol, L. V. Zinnatshina, D. N. Baleev, N. I. Sidelnikov & V. I. Ossipov

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  1. T. A. Krol
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  2. L. V. Zinnatshina
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  3. D. N. Baleev
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Correspondence to V. I. Ossipov.

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The authors declare that they have no conflicts of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

CONCLUSIONS

The composition and content of the phenolic compounds in the butanol fraction of the costmary flowers (Tanаcetum vulgare L.) which exhibited high cholagogue and anti-inflammatory activity were studied by the UPLC-PDA-HRMS method. The seven phenolic compounds were identified, including the first-discovered myricetin-3-glucoside. The content of the phenolic compounds in the butanol fraction was shown to achieve 90%. Neochlorogenic and 3,5-dicaffeoylquinic acids were found to be the main compounds (their content was as high as 69%). These results evidenced that the high content of caffeoylquinic acids could be responsible for the pharmacological activity of the medicine on the basis of the butanol fraction of the extract from the costmary flowers.

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Translated by L. Onoprienko

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Krol, T.A., Zinnatshina, L.V., Baleev, D.N. et al. An Identification and a Quantitative Assessment of Phenolic Compounds in a Butanol Fraction of Extract from Cosmary Flowers (Tanacetum vulgare). Russ J Bioorg Chem 48, 1454–1460 (2022). https://doi.org/10.1134/S1068162022070135

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