Abstract—
Mechanical forces arising in developing embryos are able to spread over considerable distances in embryos and affect gene expression. The study of mechano-dependent transcription is one of the topical issues of modern developmental biology. One of the approaches to solving this problem is the use of low-molecular-weight myosin inhibitors. In this paper, we studied by RT-PCR the effect of four myosin inhibitors—blebbistatin, S-nitroblebbistatin, ML-9 and 2,3-butanedione monoxime—on the expression of eight mechano-dependent genes in early embryos of the clawed frog. When exposed to myosin inhibitors, we observed a change in the expression level of four mechano-dependent genes, namely gsc, myh6, Xbra, and xmc. These results indicate that mechanical forces can play a role in the anterio-posterior patterning of the embryo by regulating the expression of the corresponding genes.
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This work was supported by the Russian Foundation for Basic Research (project no. 20-04-00892).
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All applicable international, national and/or institutional principles for the use of animals in experiments and the conditions for their care were observed.
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Abbreviaitons: BDM, 2,3-butanedione monooxime; RT-PCR, reverse transcription-polymerase chain reaction; MMR, Mark's modified Ringer.
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Filenko, P.A., Chechenina, A.A., Zaraisky, A.G. et al. The Effect of Myosin Inhibitors on the Expression of Mechano-Dependent Genes in the Early Development of the Clawed Frog. Russ J Bioorg Chem 48, 854–857 (2022). https://doi.org/10.1134/S1068162022040094
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DOI: https://doi.org/10.1134/S1068162022040094