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Effect of Spacers on DNA Probe Properties in Hybridization Analysis

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Abstract—

A method of immobilizing oligonucleotides on the surface of a polyethylene terephthalate (PET) substrate has been developed. The effectiveness of the complementary binding of target oligonucleotide to oligonucleotide-probes depending on the long spacers of oligonucleotide-probes that bind to the substrate was investigated. It was shown that an increase in the spacer length leads to an increase in the hybridization signal.

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ACKNOWLEDGMENTS

This work was supported by the Russian Foundation for Basic Research (project No. 20-04-00680) and a scholarship of the President of the Russian Federation for young scientists and postgraduates carrying out promising research and development in priority areas of modernization of the Russian economy for 2018–2020 (registration number SP-274.2018.4).

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Authors and Affiliations

  1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991, Moscow, Russia

    R. A. Miftakhov, S. A. Lapa, V. E. Kuznetsova, A. M. Zolotov, V. A. Vasiliskov, V. E. Shershov, S. A. Surzhikov, A. S. Zasedatelev & A. V. Chudinov

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  1. R. A. Miftakhov
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  2. S. A. Lapa
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  3. V. E. Kuznetsova
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  4. A. M. Zolotov
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  5. V. A. Vasiliskov
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  6. V. E. Shershov
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  7. S. A. Surzhikov
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  8. A. S. Zasedatelev
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  9. A. V. Chudinov
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Correspondence to A. V. Chudinov.

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The article does not contain a description of research carried out by any of the authors of this work, involving humans or using animals as objects.

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Miftakhov, R.A., Lapa, S.A., Kuznetsova, V.E. et al. Effect of Spacers on DNA Probe Properties in Hybridization Analysis. Russ J Bioorg Chem 47, 1345–1347 (2021). https://doi.org/10.1134/S1068162021060182

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  • DOI: https://doi.org/10.1134/S1068162021060182

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