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Synthesis and Antiproliferative Activity of Some New Coumarin Derivatives Derived from 8-Hydroxycoumarin

Russian Journal of Bioorganic Chemistry volume 47, pages 514–523 (2021)Cite this article

Abstract

A new series of coumarin-derived molecules have been synthesized and evaluated for their anticancer activity against human hepatocellular liver carcinoma cell line (HepG2). Compound (IX) showed the least IC50 values in MTT colorimetric assay and significantly inhibited topoisomerase IIβ. DNA flow cytometry assay of compound (IX) revealed cell cycle arrest at G2/M phase and activation of apoptosis as verified by changes in cell cycle kinetics. Further mechanism of apoptosis showed that, compound (IX) induced cell apoptosis probably through the intrinsic mitochondrial pathway of apoptosis. This mechanistic pathway was confirmed by a significant increase in the level of p53, Bax and decrease in the level of Bcl-2 compared to control.

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Authors and Affiliations

  1. Pharmaceutical Organic Chemistry Department, Faculty of Pharmacy, Port Said University, Port Said, Egypt

    Islam Zaki

  2. Chemistry Department, Faculty of Science, Port Said University, Port Said, Egypt

    El-Sherbiny H. El-Sayed & Eman M. Radwan

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  1. Islam Zaki
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Correspondence to Islam Zaki.

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Zaki, I., El-Sayed, ES.H. & Radwan, E.M. Synthesis and Antiproliferative Activity of Some New Coumarin Derivatives Derived from 8-Hydroxycoumarin. Russ J Bioorg Chem 47, 514–523 (2021). https://doi.org/10.1134/S106816202102028X

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  • DOI: https://doi.org/10.1134/S106816202102028X

Keywords:

  • coumarin
  • MTT
  • cell cycle analysis
  • annexin V
  • p53
  • Bax