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Synthesis and Optical Properties of the Conformationally Locked Indole and Indoline Derivatives of the GFP Chromophore


We report novel conformationally locked GFP chromophore amino-derivatives containing indole and indoline moieties. Optical properties of these compounds were studied. The introduction of the indoline group produces a shift in the absorption and emission maxima to the long-wavelength region and stabilizes the quantum yield of fluorescence. The introduce of the indole group leads to a hypsochromic shift and a noticeable decrease of fluorescence intensity.

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The study was carried out with the financial support of the Russian Foundation for Basic Research (project no. 18-03-00094_a).

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Correspondence to N. S. Baleeva.

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This article does not contain any research involving humans and animals as research objects.

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The authors declare no conflict of interest.

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Abbreviations: GFP, green fluorescent protein; MS, molecular sieves.

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Baleeva, N.S., Smirnov, A.Y., Myasnyanko, I.N. et al. Synthesis and Optical Properties of the Conformationally Locked Indole and Indoline Derivatives of the GFP Chromophore. Russ J Bioorg Chem 46, 862–865 (2020).

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  • imidazolones
  • chromophores
  • fluorescent dyes
  • GFP
  • optical properties