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A New Approach for the Hybridization Analysis of Pathogens

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Abstract—

The paper proposes a new variant for registering pathogens in the many-probes one-spot hybridization system. Using the example of Chlamydia trachomatis detection in blood serum samples, it has been demonstrated that hybridization of the analyzed DNA is characterized by a considerably stronger signal and higher specificity which may be advantageous for PointOfCare diagnostics (POC diagnostics).

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REFERENCES

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ACKNOWLEDGMENTS

We are grateful to M.K. Ivanov (AO Vector-Best, Novosibirsk, Russia) for facilitating this research work.

Funding

The work was supported by the Basic Research Program of the State Academies of Sciences for years 2017–2020 (grant no. VI.62.1.4, 0309-2016-0004).

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Authors and Affiliations

  1. Novosibirsk Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, 630090, Novosibirsk, Russia

    E. V. Kostina, A. N. Sinyakov & V. A. Ryabinin

Authors
  1. E. V. Kostina
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  2. A. N. Sinyakov
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  3. V. A. Ryabinin
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Correspondence to E. V. Kostina.

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Conflict of Interests

Authors declare no conflict of interest.

Statement of Compliance with Standards of Research Involving Humans as Subjects

All procedures performed in the studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants involved in the study.

Additional information

Translated by E. Martynova

Abbreviations: POC diagnostics, PointOfCare diagnostics.

Corresponding author: phone: +7 (3832) 363-51-72; e-mail: kostinaelena@mail.ru.

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Kostina, E.V., Sinyakov, A.N. & Ryabinin, V.A. A New Approach for the Hybridization Analysis of Pathogens. Russ J Bioorg Chem 45, 842–844 (2019). https://doi.org/10.1134/S1068162019060244

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  • DOI: https://doi.org/10.1134/S1068162019060244

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