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Optimization of the Technology for the Preparation of Cationic Liposomes for the Delivery of Nucleic Acids

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Abstract

Cationic liposomes based on the cationic lipid 1,26-bis(cholest-5-en-3β-yloxycarbonylamino)- 7,11,16-2-tetraazahexacosane tetrahydrochloride (2X3) and the helper lipid 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine (DOPE) have been prepared by two methods, namely ultrasonication and the extrusion of a lipid dispersion. The effect of the preparation method and the type of the water phase on the physicochemical characteristics of cationic liposomes and their biological activity has been studied. The optimization of the preparation of cationic liposomes made it possible to determine the technological parameters providing the reproducibility of physicochemical characteristics of liposomes from batch to batch.

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Abbreviations

2Х3:

1,26-bis(cholest-5-en-3β-yloxycarbonylamino)- 7,11,16-2-tetraazahexacosane tetrahydrochloride

DOPE:

1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine

EGFP:

enhanced green fluorescent protein

FBS:

fetal bovine serum

PBS:

phosphate-buffered saline, pH 7.6

siRNA:

small interfering RNA

ODN:

oligodeoxyribonucleotide

pDNA:

plasmid DNA

PAG:

polyacrylamide gel

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Authors and Affiliations

  1. Lomonosov Institute of Fine Chemical Technologies, MIREA Russian Technological University, Moscow, 119571, Russia

    A. S. Luneva, P. A. Puchkov, E. V. Shmendel & M. A. Maslov

  2. Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    M. A. Zenkova

  3. Research Center for Medical Genetics, Moscow, 115478, Russia

    A. Yu. Kuzevanova, A. A. Alimov & A. V. Karpukhin

Authors
  1. A. S. Luneva
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  2. P. A. Puchkov
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  3. E. V. Shmendel
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  4. M. A. Zenkova
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  5. A. Yu. Kuzevanova
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  6. A. A. Alimov
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  7. A. V. Karpukhin
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  8. M. A. Maslov
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Corresponding author

Correspondence to M. A. Maslov.

Additional information

Proceedings of the Conference “Lipids of XXI Century. The First Quarter,” Moscow, 22–23 October 2018.

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Luneva, A.S., Puchkov, P.A., Shmendel, E.V. et al. Optimization of the Technology for the Preparation of Cationic Liposomes for the Delivery of Nucleic Acids. Russ J Bioorg Chem 44, 724–731 (2018). https://doi.org/10.1134/S1068162019010084

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  • DOI: https://doi.org/10.1134/S1068162019010084

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