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Generation and Characterization of a Neutralizing Monoclonal Antibody Against Rabies Virus

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Abstract

Rabies is a zoonotic disease, for which effective treatment methods after the onset of clinical symptoms have not been developed yet. Polyclonal sera, both human and equine, along with vaccines are important means of disease prophylaxis. However, due to adverse reactions to the immunoglobulins of animal origin, high cost, and limited availability of the safer human serum, polyclonal antibodies should be substituted for a stable and efficient preparation, which is recombinant neutralizing antirabies monoclonal antibodies (mAbs). This paper reports generation of the humanized mAb 1C5, which binds with the antigenic site (AS) III of the rabies virus glycoprotein (RABVG) and demonstrates high virus neutralization activity in the fluorescent antibody virus neutralization test, as a result of expression in the Chinese hamster ovary (CHO) cells.

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Abbreviations

CHO:

Chinese hamster ovary cells

ERIG:

equine rabies immunoglobulin

FAVN:

fluorescent antibody virus neutralization test

HRIG:

human rabies immunoglobulin

PBS:

phosphate-buffered saline

RABV:

rabies virus

RABVG:

rabies virus glycoprotein

RIG:

rabies immunoglobulin

SOE-PCR:

splicing by overlap extension polymerase chain reaction

VH and VL:

variable domains of immunoglobulin heavy and light chains

AS:

antigenic site

mAb:

monoclonal antibodies

HP:

horseradish peroxidase

PEP:

post-exposure prophylaxis

EPR:

endoplasmic reticulum

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Authors and Affiliations

  1. Biology Faculty, Moscow State University, Moscow, 119991, Russia

    E. N. Ilina, D. A. Dolgikh & M. P. Kirpichnikov

  2. All-Russia Research Center for Molecular Diagnostics and Treatment, Moscow, 117149, Russia

    O. N. Solopova & P. G. Sveshnikov

  3. Blokhin National Medical Research Oncology Center, Ministry of Healthcare of the Russian Federation, Moscow, 115478, Russia

    O. N. Solopova

  4. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia

    D. S. Balabashin, M. V. Larina, D. A. Dolgikh & M. P. Kirpichnikov

  5. Chemistry Faculty, Moscow State University, Moscow, 119991, Russia

    T. K. Aliev

  6. Gamaleya National Research Center for Epidemiology and Microbiology, Ministry of Healthcare of the Russian Federation, Moscow, 123098, Russia

    T. V. Grebennikova, M. A. Losich & O. N. Zaykova

Authors
  1. E. N. Ilina
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  2. O. N. Solopova
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  3. D. S. Balabashin
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  4. M. V. Larina
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  8. O. N. Zaykova
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  9. P. G. Sveshnikov
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Correspondence to E. N. Ilina.

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Ilina, E.N., Solopova, O.N., Balabashin, D.S. et al. Generation and Characterization of a Neutralizing Monoclonal Antibody Against Rabies Virus. Russ J Bioorg Chem 44, 695–704 (2018). https://doi.org/10.1134/S1068162019010072

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  • DOI: https://doi.org/10.1134/S1068162019010072

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