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Efficiency of Bioluminescence Resonance Energy Transfer in the NanoLuc-miniSOG-Furimazine System

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Abstract

Photodynamic therapy (PDT) is a clinically approved, minimally invasive method for tumor destruction in the presence of a photosensitizer (PS), oxygen, and a light source. The main obstacle for the PDT treatment of deep tumors is the problem of the excitation light delivery inside the body without reduction of light intensity. An internal light source based on a bioluminescence system NanoLuc-furimazine can be considered as an alternative to an external light source. This system can be successfully used to excite a protein photosensitizer miniSOG and induce its phototoxicity in cancer cells as a result of bioluminescent resonance energy transfer. In this paper, the effectiveness of bioluminescent resonance energy transfer in the NanoLuc-miniSOG-furimazine system in living cells was studied. The efficiency of the energy transfer in this system was found to be independent of the furimazine concentration, while intracellular localization of the NanoLuc-miniSOG hybrid protein was found to be a crucial factor.

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Abbreviations

PDT:

photodynamic therapy

PS:

photosensitizer

BRET:

bioluminescence resonance energy transfer

FRET:

fluorescence resonance energy transfer

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Authors and Affiliations

  1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia

    E. I. Shramova, S. M. Deyev & G. M. Proshkina

Authors
  1. E. I. Shramova
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  2. S. M. Deyev
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  3. G. M. Proshkina
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Correspondence to E. I. Shramova.

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Original Russian Text © E.I. Shramova, S.M. Deyev, G.M. Proshkina, 2018, published in Bioorganicheskaya Khimiya, 2018, Vol. 44, No. 6, pp. 664–668.

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Shramova, E.I., Deyev, S.M. & Proshkina, G.M. Efficiency of Bioluminescence Resonance Energy Transfer in the NanoLuc-miniSOG-Furimazine System. Russ J Bioorg Chem 44, 755–758 (2018). https://doi.org/10.1134/S1068162018060080

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  • DOI: https://doi.org/10.1134/S1068162018060080

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