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Synthesis of a New Vitamin D3 Hapten and Its Protein Conjugates

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Abstract

The synthesis of 25-(1-carboxymethoxy)-imino-3(S)-hydroxy-9,10-seco-27-norcholesta-5(Z),7(E),10(19)-triene, a new hapten of vitamin D3, has been carried out in ten steps from ergosterol. The hapten was conjugated to horseradish peroxidase and bovine serum albumin. Antibodies to 25-hydroxyvitamin D3 were obtained using the synthesized conjugates. An immunochemical system was developed for the quantitative determination of 25-hydroxyvitamin D3.

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Abbreviations

25ОНD3 :

25-hydroxyvitamin D3

BSA:

bovine serum albumin

HRP:

horseradish peroxidase and PE, petroleum ether

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Authors and Affiliations

  1. Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Minsk, 220141, Belarus

    R. P. Litvinovskaya, A. P. Savachka, A. L. Sauchuk & V. A. Khripach

  2. Hemma-Test Ltd., Minsk, 220029, Belarus

    A. G. Pradko & T. V. Mirantsova

Authors
  1. R. P. Litvinovskaya
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  2. A. P. Savachka
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  3. A. L. Sauchuk
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  4. A. G. Pradko
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  5. T. V. Mirantsova
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  6. V. A. Khripach
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Corresponding author

Correspondence to R. P. Litvinovskaya.

Additional information

Original Russian Text © R.P. Litvinovskaya, A.P. Savachka, A.L. Sauchuk, A.G. Pradko, T.V. Mirantsova, V.A. Khripach, 2018, published in Bioorganicheskaya Khimiya, 2018, Vol. 44, No. 6, pp. 669–677.

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Litvinovskaya, R.P., Savachka, A.P., Sauchuk, A.L. et al. Synthesis of a New Vitamin D3 Hapten and Its Protein Conjugates. Russ J Bioorg Chem 44, 778–786 (2018). https://doi.org/10.1134/S1068162018050102

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  • DOI: https://doi.org/10.1134/S1068162018050102

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