New Synthetic Route to CY5-Labeled 2'-Deoxycytidine- 5'-Triphosphates Using Sonogashira Reaction
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Abstract
Herein, we present a new synthetic route to fluorescently-labeled nucleoside triphosphates via Sonogashira cross-coupling of iodinated deoxycytidine monophosphate and cyanine dye with a terminal alkyne group.
Keywords
alkynylated nucleoside monophosphates fluorescently labeled nucleotides indodicarbocyanine dyes Sonogashira reactionAbbreviations
- PCR
polymerase chain reaction
- DIPEA
N,Ndiisopropylethylamine
- DTDP
4,4'-dithiodipyridine
- HBTU
O-(benzotriazol-1-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate
- 5-IdCMP
5-iodo-2'-deoxycytidine-5'-monophosphate sodium salt
- Im
imidazole
- TBAP
tributylammonium pyrophosphate
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