Russian Journal of Bioorganic Chemistry

, Volume 44, Issue 2, pp 252–255 | Cite as

New Synthetic Route to CY5-Labeled 2'-Deoxycytidine- 5'-Triphosphates Using Sonogashira Reaction

  • T. O. Guseinov
  • V. E. Kuznetsova
  • V. E. Shershov
  • M. A. Spitsyn
  • S. A. Lapa
  • A. S. Zasedatelev
  • A. V. Chudinov
Letters to the Editor

Abstract

Herein, we present a new synthetic route to fluorescently-labeled nucleoside triphosphates via Sonogashira cross-coupling of iodinated deoxycytidine monophosphate and cyanine dye with a terminal alkyne group.

Keywords

alkynylated nucleoside monophosphates fluorescently labeled nucleotides indodicarbocyanine dyes Sonogashira reaction 

Abbreviations

PCR

polymerase chain reaction

DIPEA

N,Ndiisopropylethylamine

DTDP

4,4'-dithiodipyridine

HBTU

O-(benzotriazol-1-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate

5-IdCMP

5-iodo-2'-deoxycytidine-5'-monophosphate sodium salt

Im

imidazole

TBAP

tributylammonium pyrophosphate

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Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • T. O. Guseinov
    • 1
  • V. E. Kuznetsova
    • 1
  • V. E. Shershov
    • 1
  • M. A. Spitsyn
    • 1
  • S. A. Lapa
    • 1
  • A. S. Zasedatelev
    • 1
  • A. V. Chudinov
    • 1
  1. 1.Engelhardt Institute of Molecular BiologyRussian Academy of SciencesMoscowRussia

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